ENZYMATIC ASSAY FOR ADENOSYLCOBALAMIN-DEPENDENT METHYLMALONYL COENZYME-A MUTASE

被引：8
|
作者：
WATANABE, F [1 ]
TAMURA, Y [1 ]
SAIDO, H [1 ]
NAKANO, Y [1 ]
机构：
[1] UNIV OSAKA PREFECTURE, DEPT AGR CHEM, SAKAI, OSAKA 593, JAPAN
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 09期
关键词：
D O I：
10.1271/bbb.57.1593
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An enzymatic method for assaying methylmalonyl-CoA mutase was devised. The method employs a succinyl-CoA transferase and beta-hydroxyacyl-CoA dehydrogenase-coupled reaction to make the formation of NAD+ stoichiometric with the amount of the succinyl-CoA formed by the mutase. This method is one of the most convenient and useful assays for the activity of methylmalonyl-CoA mutase in biological samples.
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页码：1593 / 1594
页数：2
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