ENZYMATIC ASSAY FOR ADENOSYLCOBALAMIN-DEPENDENT METHYLMALONYL COENZYME-A MUTASE

被引：8

作者：

WATANABE, F ^{[1
]}

TAMURA, Y ^{[1
]}

SAIDO, H ^{[1
]}

NAKANO, Y ^{[1
]}

机构：

[1] UNIV OSAKA PREFECTURE, DEPT AGR CHEM, SAKAI, OSAKA 593, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 09期

关键词：

D O I：

10.1271/bbb.57.1593

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzymatic method for assaying methylmalonyl-CoA mutase was devised. The method employs a succinyl-CoA transferase and beta-hydroxyacyl-CoA dehydrogenase-coupled reaction to make the formation of NAD+ stoichiometric with the amount of the succinyl-CoA formed by the mutase. This method is one of the most convenient and useful assays for the activity of methylmalonyl-CoA mutase in biological samples.

引用

页码：1593 / 1594

页数：2

共 50 条

[1] Tritium isotope effects in adenosylcobalamin-dependent methylmalonyl-CoA mutase
Meier, TW
Thoma, NH
Leadlay, PF
BIOCHEMISTRY, 1996, 35 (36) : 11791 - 11796
[2] Protein-coenzyme interactions in adenosylcobalamin-dependent glutamate mutase
Huhta, MS
Chen, HP
Hemann, C
Hille, CR
Marsh, ENG
BIOCHEMICAL JOURNAL, 2001, 355 : 131 - 137
[3] Protection of radical intermediates at the active site of adenosylcobalamin-dependent methylmalonyl-CoA mutase
Thomä, NH
Evans, PR
Leadlay, PF
BIOCHEMISTRY, 2000, 39 (31) : 9213 - 9221
[4] Adenosylcobalamin-dependent methylmalonyl-CoA mutase isozymes in the photosynthetic protozoon Euglena gracilis Z
Watanabe, F
Abe, K
Tamura, Y
Nakano, Y
MICROBIOLOGY-SGM, 1996, 142 : 2631 - 2634
[5] Charge Separation Propensity of the Coenzyme B12-Tyrosine Complex in Adenosylcobalamin-Dependent Methylmalonyl-CoA Mutase Enzyme
Kumar, Neeraj
Liu, Shubin
Kozlowski, Pawel M.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 2012, 3 (08): : 1035 - 1038
[6] Isotope effects for deuterium transfer between substrate and coenzyme in adenosylcobalamin-dependent glutamate mutase
Cheng, MC
Marsh, ENG
BIOCHEMISTRY, 2005, 44 (07) : 2686 - 2691
[7] CRYSTALLIZATION AND PRELIMINARY DIFFRACTION DATA FOR ADENOSYLCOBALAMIN-DEPENDENT METHYLMALONYL-COA MUTASE FROM PROPIONIBACTERIUM-SHERMANII
MARSH, N
LEADLAY, PF
EVANS, PR
JOURNAL OF MOLECULAR BIOLOGY, 1988, 200 (02) : 421 - 422
[8] ALPHA-METHYLENEGLUTARATE MUTASE - AN ADENOSYLCOBALAMIN-DEPENDENT ENZYME
CHEMALY, SM
SOUTH AFRICAN JOURNAL OF CHEMISTRY-SUID-AFRIKAANSE TYDSKRIF VIR CHEMIE, 1994, 47 (02): : 37 - 47
[9] METHYLMALONYL COENZYME-A
FLAVIN, M
METHODS IN ENZYMOLOGY, 1963, 6 : 538 - 539
[10] THE SYNTHETIC SUBSTRATE SUCCINYL(CARBADETHIA)-COA GENERATES COB(II)ALAMIN ON ADENOSYLCOBALAMIN-DEPENDENT METHYLMALONYL-COA MUTASE
KEEP, NH
SMITH, GA
EVANS, MCW
DIAKUN, GP
LEADLAY, PF
BIOCHEMICAL JOURNAL, 1993, 295 : 387 - 392

← 1 2 3 4 5 →