EXPRESSION OF POTATO DAHP SYNTHASE IN ESCHERICHIA-COLI

被引：9
|
作者：
WEAVER, LM [1 ]
PINTO, JEBP [1 ]
HERRMANN, KM [1 ]
机构：
[1] PURDUE UNIV,DEPT BIOCHEM,W LAFAYETTE,IN 47907
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1993年 / 3卷 / 07期
关键词：
D O I：
10.1016/S0960-894X(01)80423-6
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Expression of a potato (Solanum tuberosum L.) cDNA encoding 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase, the first enzyme of the shikimate pathway, complemented an Escherichia coli mutant devoid of the enzyme. While the potato cDNA encodes a protein with an amino terminal putative transit sequence for chloroplast import, immunocytochemistry localized the E. coli synthesized potato enzyme to the bacterial cytosol.
引用
收藏
页码：1421 / 1428
页数：8
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