EXPRESSION OF POTATO DAHP SYNTHASE IN ESCHERICHIA-COLI

被引:9
|
作者
WEAVER, LM [1 ]
PINTO, JEBP [1 ]
HERRMANN, KM [1 ]
机构
[1] PURDUE UNIV,DEPT BIOCHEM,W LAFAYETTE,IN 47907
关键词
D O I
10.1016/S0960-894X(01)80423-6
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Expression of a potato (Solanum tuberosum L.) cDNA encoding 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase, the first enzyme of the shikimate pathway, complemented an Escherichia coli mutant devoid of the enzyme. While the potato cDNA encodes a protein with an amino terminal putative transit sequence for chloroplast import, immunocytochemistry localized the E. coli synthesized potato enzyme to the bacterial cytosol.
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页码:1421 / 1428
页数:8
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