APPLICATION OF THE SYNERGETIC LUMINESCENCE OF EU IN TIME-RESOLVED FLUOROIMMUNOASSAY

被引：6
|
作者：
LI, JJ [1 ]
CHEN, GQ [1 ]
HU, JM [1 ]
ZENG, YE [1 ]
机构：
[1] WUHAN UNIV,CTR ANAL & MEASUREMENT,WUHAN,PEOPLES R CHINA
来源：
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1992年 / 342卷 / 07期
关键词：
D O I：
10.1007/BF00321912
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The synergetic luminescence system of europium for time-resolved fluoroimmunoassay has been studied for the first time. The europium label is bound to the antibody with diethylenetriaminepentaacetic acid anhydride (DTPAA). Once the immunoreaction has been completed, the europium is preferentially dissociated from the antibody in the presence of terbium and measured by laser-induced time resolved fluorimetry. As an example, the determination of human IgG was performed. The detection limit for human immunoglobulin G was 0.25 ng/mu-l.
引用
收藏
页码：552 / 555
页数：4
相关论文
共 50 条
  • [1] APPLICATION OF SYNERGETIC LUMINESCENCE TO TIME-RESOLVED FLUOROIMMUNOASSAY
    李建军
    陈观铨
    曾云鹗
    ChineseScienceBulletin, 1992, (01) : 84 - 85
  • [2] APPLICATION OF SYNERGETIC LUMINESCENCE TO TIME-RESOLVED FLUOROIMMUNOASSAY
    LI, JJ
    CHEN, GQ
    ZENG, YE
    CHINESE SCIENCE BULLETIN, 1992, 37 (01): : 84 - 85
  • [3] Detection of Aeromonas hydrophila by Eu / Lu co-luminescence time-resolved fluoroimmunoassay
    Xie, Dongqin
    Feng, Jianjun
    Guo, Songlin
    Wang, Yilei
    Wang, Shuhong
    Lin, Peng
    Chinese Journal of Analysis Laboratory, 2021, 40 (03) : 286 - 290
  • [4] Time-resolved fluoroimmunoassay
    Degan, P
    Podestà, A
    Montagnoli, G
    MOLECULAR BIOTECHNOLOGY, 1999, 13 (03) : 215 - 222
  • [5] Time-resolved fluoroimmunoassay
    Paolo Degan
    Adriano Podestà
    Giorgio Montagnoli
    Molecular Biotechnology, 1999, 13 : 215 - 222
  • [6] TIME-RESOLVED FLUOROIMMUNOASSAY OF AFLATOXINS
    DEGAN, P
    MONTAGNOLI, G
    WILD, CP
    CLINICAL CHEMISTRY, 1989, 35 (12) : 2308 - 2310
  • [7] TIME-RESOLVED FLUOROIMMUNOASSAY OF PROGESTERONE BY EU-LABELED PROTEIN-A
    BACIGALUPO, MA
    FERRARA, L
    MERONI, G
    IUS, A
    FRESENIUS ZEITSCHRIFT FUR ANALYTISCHE CHEMIE, 1987, 328 (03): : 263 - 264
  • [8] Ultrasensitive time-resolved fluoroimmunoassay of ochratoxin A
    Huang, B
    Tao, WY
    Zhang, LF
    Shi, J
    Yang, HL
    Jin, J
    PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2005, 32 (07) : 662 - 666
  • [9] A TIME-RESOLVED FLUOROIMMUNOASSAY FOR FERRITIN IN SERUM
    CHAN, MA
    BELLEM, AC
    DIAMANDIS, EP
    CLINICAL BIOCHEMISTRY, 1987, 20 (04) : 294 - 294
  • [10] DETERMINATION OF HORMONES BY TIME-RESOLVED FLUOROIMMUNOASSAY
    LOVGREN, T
    HEMMILA, I
    PETTERSSON, K
    ESKOLA, JU
    BERTOFT, E
    TALANTA, 1984, 31 (10) : 909 - 916
12345