TIME-RESOLVED FLUOROIMMUNOASSAY OF PROGESTERONE BY EU-LABELED PROTEIN-A

被引：9
|
作者：
BACIGALUPO, MA [1 ]
FERRARA, L [1 ]
MERONI, G [1 ]
IUS, A [1 ]
机构：
[1] CNR, IST CHIM ORMONI, VIA MARIO BIANCO 9, I-20131 MILANO, ITALY
来源：
FRESENIUS ZEITSCHRIFT FUR ANALYTISCHE CHEMIE | 1987年 / 328卷 / 03期
关键词：
D O I：
10.1007/BF00481634
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A standard curve for progesterone using a solid phase time-resolved fluoroimmunoassay was obtained. The 11.alpha.-hydroxyprogesterone hemisuccinate bonded to ovalbumin was adsorbed to the wells of polystyrene microtiter strips. After incubation of scalar quantities of free progesterone (from 12 to 5000 pg) with specific antibody and an excess of Eu-labelled protein-A, the wells were washed and the europium bound to the solid phase was measured as a 2-naphthoyltrifluoroacetone chelate using a time resolved fluorometer.
引用
收藏
页码：263 / 264
页数：2
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