PURIFICATION AND PROPERTIES OF 6-PHOSPHOGLUCONATE DEHYDROGENASE FROM BEET LEAVES

被引：0
|
作者：
SIGNORINI, M [1 ]
BREGOLI, AM [1 ]
CASELLI, L [1 ]
BERGAMINI, CM [1 ]
机构：
[1] UNIV BOLOGNA,SEZ BOT,BOLOGNA,ITALY
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1995年 / 35卷 / 03期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have purified to homogeneity 6-Phosphogluconate dehydrogenase from leaves of silver beet (Beta vulgaris L.) by means of cation-exchange and affinity chromatography. The enzyme is a homodimer of 52 kDa subunits; it catalyzes NADP dependent oxidation of 6-P-gluconate with Michaelian substrate saturation. The activity is affected by some intermediates of carbohydrate metabolism, particularly erythrose-4-P. Subcellular fractionation studies indicate the cytosolic location of the enzyme.
引用
收藏
页码：669 / 675
页数：7
相关论文
共 50 条
  • [41] The Crystal Structure of 6-Phosphogluconate Dehydrogenase
    Adams, Margaret J.
    Archibald, Ian G.
    Helliwell, John R.
    Jenkins, Susan E.
    BIOCHEMICAL SOCIETY TRANSACTIONS, 1978, 6 : 1112 - 1114
  • [42] THERMOSTABILITY OF 6-PHOSPHOGLUCONATE DEHYDROGENASE FROM THERMOPHILIC FUNGI
    MILLER, HM
    CHEMISTRY IN NEW ZEALAND, 1971, 35 (04): : 129 - &
  • [43] 6-PHOSPHOGLUCONATE DEHYDROGENASE FROM BACILLUS-STEAROTHERMOPHILUS
    PEARSE, BMF
    HARRIS, JI
    FEBS LETTERS, 1973, 38 (01) : 49 - 52
  • [44] 6-PHOSPHOGLUCONATE DEHYDROGENASE FROM BACILLUS-STEAROTHERMOPHILUS
    VERONESE, FM
    BOCCU, E
    FONTANA, A
    METHODS IN ENZYMOLOGY, 1982, 89 : 282 - 291
  • [45] 6-PHOSPHOGLUCONATE DEHYDROGENASE ISOZYMES IN MAIZE
    AVERILL, RH
    KRUGER, NJ
    BAILEYSERRES, J
    PLANT PHYSIOLOGY, 1993, 102 (01) : 46 - 46
  • [46] 6-PHOSPHOGLUCONATE DEHYDROGENASE FROM SHEEP LIVER - CHEMICAL AND PHYSICAL-PROPERTIES
    LORENZEN, KE
    DYSON, JED
    FEDERATION PROCEEDINGS, 1974, 33 (05) : 1569 - 1569
  • [47] 2 ISOENZYMES EACH OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE AND 6-PHOSPHOGLUCONATE DEHYDROGENASE IN SPINACH LEAVES
    SCHNARRENBERGER, C
    OESER, A
    TOLBERT, NE
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1973, 154 (01) : 438 - 448
  • [48] ISOLATION AND SOME PROPERTIES OF 6-PHOSPHOGLUCONATE DEHYDROGENASE FROM BACILLUS-STEAROTHERMOPHILUS
    VERONESE, FM
    BOCCU, E
    FONTANA, A
    BENASSI, CA
    SCOFFONE, E
    BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 334 (01) : 31 - 44
  • [49] ENZYMOLOGICAL AND CONFORMATIONAL PROPERTIES OF 6-PHOSPHOGLUCONATE DEHYDROGENASE FROM THERMOPHILIC AND MESOPHILIC BACTERIA
    VERONESE, FM
    BOCCU, E
    FONTANA, A
    BOLLETTINO DELLA SOCIETA ITALIANA DI BIOLOGIA SPERIMENTALE, 1975, 50 (20BI): : 238 - 238
  • [50] PURIFICATION AND PROPERTIES OF GLUCOSE-6-PHOSPHATE-DEHYDROGENASE AND 6-PHOSPHOGLUCONATE DEHYDROGENASE FROM A METHANOL-UTILIZING YEAST, CANDIDA-BOIDINII
    KATO, N
    SAHM, H
    SCHUTTE, H
    WAGNER, F
    BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 566 (01) : 1 - 11
12345