PURIFICATION AND PROPERTIES OF 6-PHOSPHOGLUCONATE DEHYDROGENASE FROM BEET LEAVES

被引：0

作者：

SIGNORINI, M ^{[1
]}

BREGOLI, AM ^{[1
]}

CASELLI, L ^{[1
]}

BERGAMINI, CM ^{[1
]}

机构：

[1] UNIV BOLOGNA,SEZ BOT,BOLOGNA,ITALY

来源：

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1995年 / 35卷 / 03期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have purified to homogeneity 6-Phosphogluconate dehydrogenase from leaves of silver beet (Beta vulgaris L.) by means of cation-exchange and affinity chromatography. The enzyme is a homodimer of 52 kDa subunits; it catalyzes NADP dependent oxidation of 6-P-gluconate with Michaelian substrate saturation. The activity is affected by some intermediates of carbohydrate metabolism, particularly erythrose-4-P. Subcellular fractionation studies indicate the cytosolic location of the enzyme.

引用

页码：669 / 675

页数：7

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