COMPARISON OF THE ENZYMATIC-ACTIVITIES OF DEHYDROASCORBIC ACID REDUCTASE, GLUTATHIONE-REDUCTASE, CATALASE, PEROXIDASE AND SUPEROXIDE-DISMUTASE OF HEALTHY AND DAMAGED SPRUCE NEEDLES (PICEA-ABIES (L) KARST)

被引:51
|
作者
OSSWALD, WF [1 ]
KRAUS, R [1 ]
HIPPELI, S [1 ]
BENZ, B [1 ]
VOLPERT, R [1 ]
ELSTNER, EF [1 ]
机构
[1] TECH UNIV MUNICH,INST BOT & MIKROBIOL,W-8000 MUNICH 2,GERMANY
关键词
SPRUCE DECLINE; HYDROGEN PEROXIDE SCAVENGING ENZYMES; PIGMENT BLEACHING; ISOELECTRIC POINTS;
D O I
10.1016/S0176-1617(11)81721-9
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The stability behaviour and the recovery rates of spruce needle enzymes such as superoxide dismutase, dehydroascorbic acid reductase, glutathione reductase, catalase and peroxidase were determined. Also the isoelectric points were measured for catalase to be 8.0, for peroxidase to be 6.0 and 9.4 and for glutathione reductase to be 4.6. Furthermore, the activities of superoxide dismutase, dehydroascorbic acid reductase, glutathione reductase, catalase and peroxidase were compared for healthy and damaged spruce needles. A significant increase in enzymatic activities was measured for dehydroascorbic acid reductase glutathione reductase and catalase of damaged compared to healthy needle segments. However, no difference in enzymatic activities was found for superoxide dismutase and peroxidase of healthy and damaged needle samples.
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页码:742 / 748
页数:7
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