SEASONAL-CHANGES IN THE ACTIVITIES OF APOPLASTIC, CYTOPLASMIC, IONICALLY AND COVALENTLY BOUND ISOPEROXIDASES FROM NORWAY SPRUCE (PICEA-ABIES (L) KARST) NEEDLES - A COMPARISON BETWEEN 3 COLLECTION SITES WITH DIFFERENT AMBIENT OZONE CONCENTRATIONS

Apoplastic, cytoplasmic, ionically and covalently bound isoperoxidases were investigated from needles of Norway spruce (Picea abies (L.) Karst.) and a pronounced genetic variability of the isoenzyme pattern was found. The enzymatic activity of each group of isoperoxidases was determined between November 1989 to November 1990 from needles of three locations (Velmerstot, Glindfeld, Haltern) with different ozone concentrations. Apoplastic peroxidases of needles from trees grown in Velmerstot and Glindfeld showed up to two-fold higher activities than the same group of peroxidases from Haltern. Based on the recorded increased ozone concentrations in Velmerstot and Glindfeld a positive correlation between the activity of the apoplastic peroxidases and ambient ozone concentrations at the different locations could be demonstrated. A putative role of the apoplastic peroxidases as a bioindicator for air pollutants is discussed.