GENOMIC ANALYSIS OF A MOUSE ZINC FINGER GENE, ZFP-35, THAT IS UP-REGULATED DURING SPERMATOGENESIS

Zinc finger genes are a class of eukaryotic regulatory genes that encode sequence-specific nucleic acid-binding proteins. Members of this large gene family are required for growth and development in a wide range of organisms. We previously identified a mouse zinc finger gene, Zfp-35, that was up-regulated during spermatogenesis at the pachytene spermatocyte stage of development. We now describe the genomic organization of this gene, including its intron-exon structure, the sequence of its flanking regions, and its assignment to a region encompassing bands B3 to C of chromosome 18. The transcription unit has three exons. Intron 1 is within the 5′ untranslated region and exon 3 contains the block of all 18 zinc fingers. These two features are common to a number of zinc finger genes. We also show that Zfp-35 is conserved in some placental mammals and that it is a member of a subfamily of related mammalian zinc finger genes. © 1990.