NUCLEIC-ACID HYBRIDIZATION STUDIES WITH SWEDISH BYDV ISOLATES

被引：0
|
作者：
OXELFELT, P
EWEIDA, M
机构：
来源：
ACTA PHYTOPATHOLOGICA ET ENTOMOLOGICA HUNGARICA | 1991年 / 26卷 / 1-2期
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D O I：
暂无
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
RNA of a Swedish BYDV isolate of the MAV type was used as template for the synthesis of cDNA which was cloned in plasmic pUC19. Some clones hybridize specifically with MAV-type isolates, whereas others hybridize with both MAV- and PAV-like isolates. P-32-labelled probes readily detect BYDV in plant sap and in extracts from single viruliferous aphids in filter hybridization tests. Biotinylated probes detect BYDV in sap samples of oats prepared by a simple procedure without background problems.
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页码：9 / 13
页数：5
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