NUCLEIC-ACID HYBRIDIZATION STUDIES WITH SWEDISH BYDV ISOLATES

被引：0
|
作者：
OXELFELT, P
EWEIDA, M
机构：
来源：
ACTA PHYTOPATHOLOGICA ET ENTOMOLOGICA HUNGARICA | 1991年 / 26卷 / 1-2期
关键词：
D O I：
暂无
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
RNA of a Swedish BYDV isolate of the MAV type was used as template for the synthesis of cDNA which was cloned in plasmic pUC19. Some clones hybridize specifically with MAV-type isolates, whereas others hybridize with both MAV- and PAV-like isolates. P-32-labelled probes readily detect BYDV in plant sap and in extracts from single viruliferous aphids in filter hybridization tests. Biotinylated probes detect BYDV in sap samples of oats prepared by a simple procedure without background problems.
引用
收藏
页码：9 / 13
页数：5
相关论文
共 50 条
  • [21] NUCLEIC-ACID SANDWICH HYBRIDIZATION IN ADENOVIRUS DIAGNOSIS
    RANKI, M
    VIRTANEN, M
    PALVA, A
    LAAKSONEN, M
    PETTERSSON, R
    KAARIAINEN, L
    HALONEN, P
    SODERLUND, H
    CURRENT TOPICS IN MICROBIOLOGY AND IMMUNOLOGY, 1983, 104 : 307 - 318
  • [22] NUCLEIC-ACID HYBRIDIZATION FOR THE DETECTION OF VIRAL GENOMES
    BORNKAMM, GW
    DESGRANGES, C
    GISSMANN, L
    CURRENT TOPICS IN MICROBIOLOGY AND IMMUNOLOGY, 1983, 104 : 287 - 298
  • [23] NUCLEIC-ACID HYBRIDIZATION METHODS TO DETECT MICROORGANISMS
    GRAVITT, PE
    MANOS, MM
    LABORATORY ANIMAL SCIENCE, 1993, 43 (01): : 5 - 10
  • [24] NUCLEIC-ACID HYBRIDIZATION - ITS APPLICATION TO DIAGNOSIS
    BARCELONA, SA
    INTERFERON Y BIOTECNOLOGIA, 1988, 5 (03): : 195 - 209
  • [25] NUCLEIC-ACID HYBRIDIZATION DETECTED BY PIEZOELECTRIC RESONANCE
    FAWCETT, NC
    EVANS, JA
    CHIEN, LC
    FLOWERS, N
    ANALYTICAL LETTERS, 1988, 21 (07) : 1099 - 1114
  • [26] IDENTIFICATION OF FOODBORNE PATHOGENS BY NUCLEIC-ACID HYBRIDIZATION
    HILL, WE
    KEASLER, SP
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1991, 12 (01) : 67 - 75
  • [27] MICROBIAL DIAGNOSIS BY NUCLEIC-ACID SANDWICH HYBRIDIZATION
    PALVA, A
    RANKI, M
    CLINICS IN LABORATORY MEDICINE, 1985, 5 (03) : 475 - 490
  • [28] NUCLEIC-ACID HYBRIDIZATION - TRIPLEX STABILITY AND ENERGETICS
    PLUM, GE
    PILCH, DS
    SINGLETON, SF
    BRESLAUER, KJ
    ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 1995, 24 : 319 - 350
  • [29] APPLICATIONS OF NUCLEIC-ACID HYBRIDIZATION IN MICROBIAL ECOLOGY
    HOLBEN, WE
    TIEDJE, JM
    ECOLOGY, 1988, 69 (03) : 561 - 568
  • [30] BIOCHEMICAL AND NUCLEIC-ACID HYBRIDIZATION STUDIES ON BREVIBACTERIUM-LINENS AND RELATED STRAINS
    FIEDLER, F
    SCHAFFLER, MJ
    STACKEBRANDT, E
    ARCHIVES OF MICROBIOLOGY, 1981, 129 (01) : 85 - 93
12345