A SPHINGOMYELIN-TRANSFERRING PROTEIN FROM CHICKEN LIVER - USE OF PYRENE-LABELED PHOSPHOLIPID

被引：29

作者：

TIMMERMANSHEREIJGERS, JLPM

SNOEK, GT

WIRTZ, KWA

机构：

[1] UNIV UTRECHT,CTR BIOMEMBRANES & LIPID ENZYMOL,3508 TB UTRECHT,NETHERLANDS

[2] HELSINKI UNIV,DEPT BASIC CHEM,SF-00014 HELSINKI,FINLAND

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 24期

关键词：

D O I：

10.1074/jbc.270.24.14263

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A phospholipid transfer protein was purified from chicken liver which, in addition to phosphatidylinositol (PI) and phosphatidylcholine (PC), carries sphingomyelin (SM) between membranes. For comparison, the PI-transfer protein from chicken liver only carries PI and PC. Specificity was established by use of phospholipids that carry a pyrene-labeled acyl chain. Based on the N-terminal sequence and Western blot analysis we conclude that this protein is an isoform of the PI-transfer protein. At increasing length of the pyrene-labeled acyl chain, the isoform expresses a high activity toward SM, a low activity toward PI, and virtually no activity toward PC.

引用

页码：14263 / 14266

页数：4

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