A SPHINGOMYELIN-TRANSFERRING PROTEIN FROM CHICKEN LIVER - USE OF PYRENE-LABELED PHOSPHOLIPID

被引：29
|
作者：
TIMMERMANSHEREIJGERS, JLPM
SNOEK, GT
WIRTZ, KWA
机构：
[1] UNIV UTRECHT,CTR BIOMEMBRANES & LIPID ENZYMOL,3508 TB UTRECHT,NETHERLANDS
[2] HELSINKI UNIV,DEPT BASIC CHEM,SF-00014 HELSINKI,FINLAND
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 24期
关键词：
D O I：
10.1074/jbc.270.24.14263
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A phospholipid transfer protein was purified from chicken liver which, in addition to phosphatidylinositol (PI) and phosphatidylcholine (PC), carries sphingomyelin (SM) between membranes. For comparison, the PI-transfer protein from chicken liver only carries PI and PC. Specificity was established by use of phospholipids that carry a pyrene-labeled acyl chain. Based on the N-terminal sequence and Western blot analysis we conclude that this protein is an isoform of the PI-transfer protein. At increasing length of the pyrene-labeled acyl chain, the isoform expresses a high activity toward SM, a low activity toward PI, and virtually no activity toward PC.
引用
收藏
页码：14263 / 14266
页数：4
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