OPTIMIZATION OF INFLUENZA A AND B VIRUSES CULTIVATION CONDITIONS FOR PREPARATION OF TRIVALENT SEASONAL INFLUENZA SPLIT VACCINE

被引：0
|
作者：
Akzhunusova, Indira [1 ]
Assanzhanova, Nurika [1 ]
Tabynov, Kaissar [1 ]
Abdrakhmanova, Buryl [2 ]
Sansyzbay, Abilai [1 ]
机构：
[1] Res Inst Biol Safety Problems, Gvardeisky 080409, Kazakhstan
[2] Kyrgyz State Univ, Bishkek, Kyrgyzstan
来源：
INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH | 2015年 / 6卷 / 06期
关键词：
Influenza A and B viruses; cultivation conditions; chicken embryos; seasonal influenza vaccine;
D O I：
10.13040/IJPSR.0975-8232.6(6).2375-81
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
This article presents the results of studies on determination of optimal parameters of recombinant strains A/NYMC X-217 (H3N2) and B/NYMC BX-49 of influenza virus cultivation in chicken embryos. It is found that a maximum virus accumulation is formed under the following cultivation conditions: age of chicken embryos is 9-11 days in case of inoculation of the allantoic cavity, inoculated virus dose - 1,000-10,000 EID50, temperature and time of incubation - (34 +/- 0.5) degrees C and 48 h, respectively. If the cultivation parameters are satisfied, it is possible to obtain a highly active viruscontaining material with infectious and hemagglutinating activity, as well as a content of hemagglutinin not less than 7.0lg EID50/ml, 1: 256 and 7.0 mu g/ml, respectively, that is enough to prepare a trivalent inactive seasonal influenza split vaccine.
引用
收藏
页码：2375 / 2381
页数：7
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