EXPRESSION OF CHIMERIC PROTEINS ENCODED BY THE FUSED BLV REVERSE-TRANSCRIPTASE - BETA-GALACTOSIDASE IN ESCHERICHIA-COLI

被引：0
|
作者：
SUPEK, F [1 ]
PECHAN, T [1 ]
TURNA, J [1 ]
机构：
[1] COMENIUS UNIV, FAC NAT SCI, DEPT MOLEC BIOL, CS-84215 BRATISLAVA, CZECHOSLOVAKIA
来源：
FOLIA BIOLOGICA | 1992年 / 38卷 / 05期
关键词：
BOVINE LEUKEMIA VIRUS; REVERSE TRANSCRIPTASE; GENE FUSION; HETEROLOGOUS EXPRESSION;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The gene for bovine leukemia virus (BLV) reverse transcriptase was cloned in prokaryotic expression vector pUC8-2. After fusion of Escherichia coli lacZ gene to different parts of reverse transcriptase we detected expression of new proteins with molecular weights corresponding to the size of the hybrid genes. A coding region most probably responsible for about a hundred-fold decrease in expression of long fusion proteins has been identified. A few possible causes of this phenomenon were tested.
引用
收藏
页码：277 / 283
页数：7
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