EXPRESSION OF CHIMERIC PROTEINS ENCODED BY THE FUSED BLV REVERSE-TRANSCRIPTASE - BETA-GALACTOSIDASE IN ESCHERICHIA-COLI

被引：0

作者：

SUPEK, F ^{[1
]}

PECHAN, T ^{[1
]}

TURNA, J ^{[1
]}

机构：

[1] COMENIUS UNIV, FAC NAT SCI, DEPT MOLEC BIOL, CS-84215 BRATISLAVA, CZECHOSLOVAKIA

来源：

FOLIA BIOLOGICA | 1992年 / 38卷 / 05期

关键词：

BOVINE LEUKEMIA VIRUS; REVERSE TRANSCRIPTASE; GENE FUSION; HETEROLOGOUS EXPRESSION;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene for bovine leukemia virus (BLV) reverse transcriptase was cloned in prokaryotic expression vector pUC8-2. After fusion of Escherichia coli lacZ gene to different parts of reverse transcriptase we detected expression of new proteins with molecular weights corresponding to the size of the hybrid genes. A coding region most probably responsible for about a hundred-fold decrease in expression of long fusion proteins has been identified. A few possible causes of this phenomenon were tested.

引用

页码：277 / 283

页数：7

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