THE SELF-CLEAVAGE OF LARIAT-RNA

被引：24

作者：

AGBACK, P ^{[1
]}

GLEMAREC, C ^{[1
]}

YIN, L ^{[1
]}

SANDSTROM, A ^{[1
]}

PLAVEC, J ^{[1
]}

SUND, C ^{[1
]}

YAMAKAGE, S ^{[1
]}

VISWANADHAM, G ^{[1
]}

ROUSSE, B ^{[1
]}

PURI, N ^{[1
]}

CHATTOPADHYAYA, J ^{[1
]}

机构：

[1] UNIV UPPSALA,CTR BIOMED,DEPT BIOORGAN CHEM,BOX 581,S-75123 UPPSALA,SWEDEN

来源：

TETRAHEDRON LETTERS | 1993年 / 34卷 / 24期

关键词：

D O I：

10.1016/S0040-4039(00)79266-5

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Lariat-RNAs 3 and 4 undergo site-specific self-cleavage reaction at the G3 --> C6/U7 phosphodiester bond by the nucleophilic attack of 2'-OH of G3 sugar moiety to its 3-phosphate to give 5'hydroxyl terminal at C6 or U7 and 2, 3-cyclic phosphodiester of G3 whereas lariat-tetramer 1, pentamer 2, the cyclic-A(2' --> 5)G-tetramer 5 and the cyclic-A(3' --> 5')G-tetramer 6 are completely stable. The lariat-RNAs 3 and 4 are the smallest RNA known to undergo self-cleavage which is reminiscent of the RNA-hammerhead (Ribozyme) activity. The geometry of the cleavage-site in 3 and 4 has been defined by full conformational analysis by NMR and molecular dynamics calculation in water.

引用

页码：3929 / 3932

页数：4

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