Separation of Etoricoxib and Its Degradation Products in Drug Substance Using UPLC (TM)

被引：6
|
作者：
Vora, Dinesh N. [1 ]
Kadav, Arun A.
机构：
[1] Mithibai Coll Arts, Chauhan Inst Sci, Chem Dept, Viile Parle W, Bombay 400056, Maharashtra, India
来源：
EURASIAN JOURNAL OF ANALYTICAL CHEMISTRY | 2007年 / 2卷 / 03期
关键词：
Etoricoxib; degradation products; UPLC; validation;
D O I：
10.12973/ejac.2007.00019a
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A UPLC (TM) method was developed and validated for the separation of Etoricoxib and its degradation products in drug substance. The main drug and its seven major and few minor degradation products were well separated within seven minutes on acquity UPLC (TM) BEH C18 column (1.7 mu, 2.1 x 100 mm) maintained at 25 degrees C, using 0.01M acetate buffer pH 5.0 - acetonitrile (60 : 40, v/v) as mobile phase. The flow rate was 0.3 mL.min(-1) and observed backpressure was about 10500 psi. Detection was performed at 235 nm using PDA detector. Linearity was obtained in the concentration range of 0.05 - 120 mu g.mL(-1). The method was validated for precision, linearity, LOD and LOQ. Stability indicating capability was established by forced degradation experiments.
引用
收藏
页码：151 / 158
页数：8
相关论文
共 50 条
  • [41] Separation of oxytocin degradation products using capillary electrophoresis with UV detection
    Krauss, Shannon T.
    Creamer, Jessica S.
    Lunte, Susan M.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2013, 245
  • [42] Characterization of stress degradation products of curcumin and its two derivatives by UPLC-DAD-MS/MS
    Wang, Linli
    Lu, Nan
    Zhao, Longshan
    Qi, Chao
    Zhang, Weiyang
    Dong, Jinhua
    Hou, Xiaohong
    ARABIAN JOURNAL OF CHEMISTRY, 2019, 12 (08) : 3998 - 4005
  • [43] Enantiomeric separation of a drug substance using capillary electrophoresis with sulfated-β-cyclodextrin
    Yuan, HM
    Thompson, RA
    Ellison, DK
    JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2002, 25 (19) : 2999 - 3015
  • [44] Separation and detection of VX and its methylphosphonic acid degradation products on a microchip using indirect laser-induced fluorescence
    Heleg-Shabtai, Vered
    Gratziany, Natzach
    Liron, Zvi
    ELECTROPHORESIS, 2006, 27 (10) : 1996 - 2001
  • [45] SEPARATION OF PENICILLIN-G POTASSIUM AND ITS DEGRADATION PRODUCTS USING HIGH-PRESSURE LIQUID-CHROMATOGRAPHY
    VADINO, WA
    SUGITA, ET
    SCHNAARE, RL
    ANDO, HY
    NIEBERGALL, PJ
    JOURNAL OF PHARMACEUTICAL SCIENCES, 1979, 68 (10) : 1316 - 1318
  • [46] Separation and identification of neuropeptide Y, two of its fragments and their degradation products using capillary electrophoresis-mass spectrometry
    Ensing, Kees
    de Boer, Theo
    Schreuder, Nanno
    de Zeeuw, Rokus A.
    Journal of Chromatography B, 1999, 727 (01): : 53 - 61
  • [47] Validated UPLC and TLC-Densitometry Stability Indicating Methods for the Determination of Rafoxanide in the Presence of Its Degradation Products
    Saad, Ahmed S.
    Hamdy, Abdallah M.
    Salama, Fathy M.
    Abdelkawy, Mohamed
    JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2016, 54 (09) : 1661 - 1669
  • [48] DEVELOPMENT AND VALIDATION OF STABILITY-INDICATING UPLC METHOD FOR THE DETERMINATION OF BRIVARACETAM, ITS RELATED IMPURITIES AND DEGRADATION PRODUCTS
    Vishweshwar, Vavilala
    Babu, J. Moses
    Muralikrishna, R.
    INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH, 2018, 9 (06): : 2315 - 2327
  • [49] Fractional factorial design optimization of the separation of pilocarpine and its degradation products by capillary electrophoresis
    Persson, K
    Astrom, O
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1997, 697 (1-2): : 207 - 215
  • [50] DETERMINATION AND QUANTITATION OF BENDROFLUMETHIAZIDE AND ITS DEGRADATION PRODUCTS USING HPLC
    FRONTINI, R
    MIELCK, JB
    JOURNAL OF LIQUID CHROMATOGRAPHY, 1992, 15 (14): : 2519 - 2528
12345