RPLC ANALYSIS OF ALKYL POLYETHYLENEGLYCOL ETHERS USING EVAPORATIVE LIGHT-SCATTERING DETECTION

被引:0
|
作者
GUERRERO, F
ROCCA, JL
机构
[1] UNIV LYON 1, SCI ANALYT LAB, CNRS, UA 435, F-69622 VILLEURBANNE, FRANCE
[2] SERV CENT ANAL ECHANGEUR SOLAIZE, F-69390 VERNAISON, FRANCE
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D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A chromatographic method is described for the RPLC analysis of complex mixtures of alkyl polyethyleneglycol ethers. Solutes are separated according to both their hydrophobic and hydrophilic chains, in gradient elution mode using acetonitrile / water binary mobile phases. The interest of the evaporative light scattering detection is discussed for the quantitative analysis of such solutes. Study of detector response and optimization of working conditions made it possible to obtain good reproducibility of analyses and interesting detection limit of the chromatographic method.
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页码:11 / 15
页数:5
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