THROMBIN STIMULATES THE PRODUCTION OF IMMUNOREACTIVE ENDOTHELIN-1 IN CULTURED HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS

被引：41
|
作者：
KOHNO, M
YASUNARI, K
YOKOKAWA, K
MURAKAWA, K
HORIO, T
KANAYAMA, Y
FUZISAWA, M
INOUE, T
TAKEDA, T
机构：
[1] First Department of Internal Medicine, Osaka City University Medical School, Abeno-ku, Osaka, Asahimachi
来源：
METABOLISM-CLINICAL AND EXPERIMENTAL | 1990年 / 39卷 / 10期
关键词：
D O I：
10.1016/0026-0495(90)90157-8
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The present study was designed to examine the effect of thrombin on endothelin production by cultured human umbilical vein endothelial cells (HUVEC) by radioimmunoassay. Cultured HUVEC released immunoreactive endothelin-1 (ir-endothelin-1) into the medium in a time-dependent manner. This release was inhibited completely by 10 μg/mL cycloheximide and is, therefore, directly related to de novo protein synthesis. Further endothelin release was stimulated by the addition of thrombin to the culture medium. Thrombin-induced ir-endothelin-1 release also was inhibited completely by 10 μg/mL cycloheximide. These results suggest that HUVEC in culture produce ir-endothelin-1 slowly, but continuously, and that this production is stimulated by thrombin. © 1990.
引用
收藏
页码：1003 / 1005
页数：3
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