THROMBIN STIMULATES THE PRODUCTION OF IMMUNOREACTIVE ENDOTHELIN-1 IN CULTURED HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS

被引：41
|
作者：
KOHNO, M
YASUNARI, K
YOKOKAWA, K
MURAKAWA, K
HORIO, T
KANAYAMA, Y
FUZISAWA, M
INOUE, T
TAKEDA, T
机构：
[1] First Department of Internal Medicine, Osaka City University Medical School, Abeno-ku, Osaka, Asahimachi
来源：
METABOLISM-CLINICAL AND EXPERIMENTAL | 1990年 / 39卷 / 10期
关键词：
D O I：
10.1016/0026-0495(90)90157-8
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The present study was designed to examine the effect of thrombin on endothelin production by cultured human umbilical vein endothelial cells (HUVEC) by radioimmunoassay. Cultured HUVEC released immunoreactive endothelin-1 (ir-endothelin-1) into the medium in a time-dependent manner. This release was inhibited completely by 10 μg/mL cycloheximide and is, therefore, directly related to de novo protein synthesis. Further endothelin release was stimulated by the addition of thrombin to the culture medium. Thrombin-induced ir-endothelin-1 release also was inhibited completely by 10 μg/mL cycloheximide. These results suggest that HUVEC in culture produce ir-endothelin-1 slowly, but continuously, and that this production is stimulated by thrombin. © 1990.
引用
收藏
页码：1003 / 1005
页数：3
相关论文
共 50 条
  • [21] THROMBIN-INDUCED VIMENTIN PHOSPHORYLATION IN CULTURED HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS
    BORMANN, BJ
    HUANG, CK
    LAM, GF
    JAFFE, EA
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1986, 261 (23) : 471 - 477
  • [22] SECRETION OF PROSTAGLANDINS AND ENDOTHELIN-1 BY DECIDUAL ENDOTHELIAL-CELLS FROM NORMAL AND PREECLAMPTIC PREGNANCIES - COMPARISON WITH HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS
    GALLERY, EDM
    ROWE, J
    CAMPBELL, S
    HAWKINS, T
    AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1995, 173 (05) : 1557 - 1562
  • [23] BRADYKININ STIMULATES INOSITOL PHOSPHATE ACCUMULATION IN CULTURED HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS
    LO, WWY
    FAN, TPD
    MEDICAL SCIENCE RESEARCH-BIOCHEMISTRY, 1987, 15 (21-22): : 1325 - 1325
  • [24] METALLOTHIONEIN INDUCTION BY CADMIUM, CYTOKINES, THROMBIN AND ENDOTHELIN-1 IN CULTURED VASCULAR ENDOTHELIAL-CELLS
    KAJI, T
    YAMAMOTO, C
    TSUBAKI, S
    OHKAWARA, S
    SAKAMOTO, M
    SATO, M
    KOZUKA, H
    LIFE SCIENCES, 1993, 53 (15) : 1185 - 1191
  • [25] INTERLEUKIN-1 INCREASES THE PRODUCTION OF ENDOTHELIN-1 BY CULTURED ENDOTHELIAL-CELLS
    YOSHIZUMI, M
    KURIHARA, H
    MORITA, T
    YAMASHITA, T
    OHHASHI, Y
    SUGIYAMA, T
    TAKAKU, F
    YANAGISAWA, M
    MASAKI, T
    YAZAKI, Y
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 166 (01) : 324 - 329
  • [26] Specificity of glycosaminoglycan suppression of endothelin-1 production by human umbilical vein endothelial cells.
    Valencia, AO
    Mileva, M
    Dweck, HS
    Rosenfeld, L
    JOURNAL OF INVESTIGATIVE MEDICINE, 1998, 46 (01) : 177A - 177A
  • [27] Effect of endotoxin on the celluar activity and secretion of endothelin-1 in cultured human umbilical vein endothelial cells
    中国病理生理杂志, 2001, (08) : 80 - 81
  • [28] ENDOTHELIN-1 AND THROMBIN SYNERGISTICALLY STIMULATE IL-6 MESSENGER-RNA EXPRESSION AND PROTEIN-PRODUCTION IN HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS
    STANKOVA, J
    ROLAPLESZCZYNSKI, M
    DORLEANSJUSTE, P
    JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 1995, 26 : S505 - S507
  • [29] Thrombin stimulates production of interleukin-8 in human umbilical vein endothelial cells
    Ueno, A
    Murakami, K
    Yamanouchi, K
    Watanabe, M
    Kondo, T
    IMMUNOLOGY, 1996, 88 (01) : 76 - 81
  • [30] POLAR SECRETION OF ENDOTHELIN-1 BY CULTURED ENDOTHELIAL-CELLS
    WAGNER, OF
    CHRIST, G
    WOJTA, J
    VIERHAPPER, H
    PARZER, S
    NOWOTNY, PJ
    SCHNEIDER, B
    WALDHAUSL, W
    BINDER, BR
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1992, 267 (23) : 16066 - 16068
12345