Biosensor based on a glassy carbon electrode modified with tyrosinase immmobilized on multiwalled carbon nanotubes

被引：1
|
作者：
Jing Ren
Tian-Fang Kang
Rui Xue
Chao-Nan Ge
Shui-Yuan Cheng
机构：
[1] Beijing University of Technology,College of Environmental and Energy Engineering
来源：
Microchimica Acta | 2011年 / 174卷
关键词：
Tyrosinase; Multiwalled carbon nanotubes; Electrochemical biosensors; Phenols;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We describe a biosensor for phenolic compounds that is based on a glassy carbon electrode modified with tyrosinase immobilized on multiwalled carbon nanotubes (MWNTs). The MWNTs possess excellent inherent electrical conductivity which enhances the electron transfer rate and results in good electrochemical catalytic activity towards the reduction of benzoquinone produced by enzymatic reaction. The biosensor was characterized by cyclic voltammetry, and the experimental conditions were optimized. The cathodíc current is linearly related to the concentration of the phenols between 0.4 μM and 10 μM, and the detection limit is 0.2 μM. The method was applied to the determination of phenol in water samples.
引用
收藏
页码：303 / 309
页数：6
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