Genetic improvement of tomato by targeted control of fruit softening

被引：262

作者：

Uluisik, Selman ^{[1
]}

Chapman, Natalie H. ^{[1
]}

Smith, Rebecca ^{[1
,11
]}

Poole, Mervin ^{[2
]}

Adams, Gary ^{[1
,3
]}

Gillis, Richard B. ^{[1
,3
]}

Besong, Tabot M. D. ^{[1
,12
]}

Sheldon, Judith ^{[4
]}

Stiegelmeyer, Suzy ^{[5
,13
]}

Perez, Laura ^{[6
]}

Samsulrizal, Nurul ^{[1
]}

Wang, Duoduo ^{[1
]}

Fisk, Ian D. ^{[1
]}

Yang, Ni ^{[1
]}

Baxter, Charles ^{[4
]}

Rickett, Daniel ^{[4
]}

Fray, Rupert ^{[1
]}

Blanco-Ulate, Barbara ^{[7
]}

Powell, Ann L. T. ^{[7
]}

Harding, Stephen E. ^{[1
]}

Craigon, Jim ^{[1
]}

Rose, Jocelyn K. C. ^{[8
]}

Fich, Eric A. ^{[8
]}

Sun, Li ^{[9
,10
]}

Domozych, David S. ^{[9
,10
]}

Fraser, Paul D. ^{[6
]}

Tucker, Gregory A. ^{[1
]}

Grierson, Don ^{[1
]}

Seymour, Graham B. ^{[1
]}

机构：

[1] Univ Nottingham, Sch Biosci, Sutton Bonington Campus, Loughborough, Leics, England

[2] Bugbrooke Mills, Heygates Ltd, Bugbrooke, England

[3] Univ Nottingham, Sch Hlth Sci, Queens Med Ctr, Nottingham, England

[4] Jealotts Hill Int Res Stn, Syngenta Seeds, Bracknell, Berks, England

[5] Syngenta Crop Protect, Res Triangle Pk, NC USA

[6] Royal Holloway Univ London, Sch Biol Sci, Egham Hill, Egham, Surrey, England

[7] Univ Calif Davis, Plant Sci Dept, Davis, CA 95616 USA

[8] Cornell Univ, Plant Biol Sect, Sch Integrat Plant Sci, Ithaca, NY 14853 USA

[9] Skidmore Coll, Dept Biol, Saratoga Springs, NY 12866 USA

[10] Skidmore Coll, Skidmore Microscopy Imaging Ctr, Saratoga Springs, NY 12866 USA

[11] Springalls Farm, Valley Produce, Reading, Berks, England

[12] King Abdullah Univ Sci & Technol, Phys Sci & Engn Div, Thuwal, Saudi Arabia

[13] Q2 Solut, EA Genom, Morrisville, NC USA

来源：

NATURE BIOTECHNOLOGY | 2016年 / 34卷 / 09期

基金：

英国生物技术与生命科学研究理事会; 美国国家科学基金会; 英国工程与自然科学研究理事会;

关键词：

LYCOPERSICON-ESCULENTUM; POLYGALACTURONASE GENE; FRESH TOMATO; CELL-WALLS; EXPRESSION; HOMOGALACTURONAN; INHIBITION; REDUCTION;

D O I：

10.1038/nbt.3602

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Controlling the rate of softening to extend shelf life was a key target for researchers engineering genetically modified (GM) tomatoes in the 1990s, but only modest improvements were achieved. Hybrids grown nowadays contain 'non-ripening mutations' that slow ripening and improve shelf life, but adversely affect flavor and color. We report substantial, targeted control of tomato softening, without affecting other aspects of ripening, by silencing a gene encoding a pectate lyase.

引用

页码：950 / +

页数：6

共 50 条

[31] The genetic basis and improvement of photosynthesis in tomato
Haiqiang Dong
Fangman Li
Xiaoxiao Xuan
John Kojo Ahiakpa
Jinbao Tao
Xingyu Zhang
Pingfei Ge
Yaru Wang
Wenxian Gai
Yuyang Zhang
Horticultural Plant Journal, 2025, 11 (01) : 69 - 84
[32] Genetic challenges of flavor improvement in tomato
Klee, Harry J.
Tieman, Denise M.
TRENDS IN GENETICS, 2013, 29 (04) : 257 - 262
[33] Genetic Analysis of Fruit Ripening and Quality in Tomato
Barry, Cornelius
HORTSCIENCE, 2010, 45 (08) : S40 - S41
[34] Genetic and Proteomic Approach of Tomato Fruit Quality
Faurobert, M.
Chaib, J.
Barre, M.
Tricon, D.
Munos, S.
Causse, M.
I INTERNATIONAL SYMPOSIUM ON HORTICULTURE IN EUROPE, 2009, 817 : 119 - 126
[35] Genetic engineering of parthenocarpic fruit development in tomato
Nadia Ficcadenti
Sara Sestili
Tiziana Pandolfini
Chiara Cirillo
Giuseppe Leonardo Rotino
Angelo Spena
Molecular Breeding, 1999, 5 : 463 - 470
[36] Gibberellins involved in fruit ripening and softening by mediating multiple hormonal signals in tomato
Wu, Mengbo
Liu, Kaidong
Li, Honghai
Li, Ying
Zhu, Yunqi
Su, Dan
Zhang, Yaoxin
Deng, Heng
Wang, Yikui
Liu, Mingchun
HORTICULTURE RESEARCH, 2024, 11 (02)
[37] Genome Editing of Tomato with CRISPR/Cas 9 Targeting Fruit Softening Genes
Boase, Murray
Tiffin, Heather
Zhang, Huaibi
Hunter, Donald
Erridge, Zoe
Wang, Lei
Mandhandhar, Sarina
King, Ian
Napier, Nathanael
Brummell, David
Schaffer, Robert
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 2019, 55 : S58 - S58
[38] GENETIC-REGULATION OF TOMATO FRUIT RIPENING
TIGCHELAAR, EC
MCGLASSON, WB
BUESCHER, RW
HORTSCIENCE, 1978, 13 (05) : 508 - 513
[39] Genetic engineering of parthenocarpic fruit development in tomato
Ficcadenti, N
Sestili, S
Pandolfini, T
Cirillo, C
Rotino, GL
Spena, A
MOLECULAR BREEDING, 1999, 5 (05) : 463 - 470
[40] Chilling-associated softening of tomato fruit is related to increased pectinmethylesterase activity
Marangoni, AG
Jackman, RL
Stanley, DW
JOURNAL OF FOOD SCIENCE, 1995, 60 (06) : 1277 - 1281

← 1 2 3 4 5 →