Genetic improvement of tomato by targeted control of fruit softening

被引：262

作者：

Uluisik, Selman ^{[1
]}

Chapman, Natalie H. ^{[1
]}

Smith, Rebecca ^{[1
,11
]}

Poole, Mervin ^{[2
]}

Adams, Gary ^{[1
,3
]}

Gillis, Richard B. ^{[1
,3
]}

Besong, Tabot M. D. ^{[1
,12
]}

Sheldon, Judith ^{[4
]}

Stiegelmeyer, Suzy ^{[5
,13
]}

Perez, Laura ^{[6
]}

Samsulrizal, Nurul ^{[1
]}

Wang, Duoduo ^{[1
]}

Fisk, Ian D. ^{[1
]}

Yang, Ni ^{[1
]}

Baxter, Charles ^{[4
]}

Rickett, Daniel ^{[4
]}

Fray, Rupert ^{[1
]}

Blanco-Ulate, Barbara ^{[7
]}

Powell, Ann L. T. ^{[7
]}

Harding, Stephen E. ^{[1
]}

Craigon, Jim ^{[1
]}

Rose, Jocelyn K. C. ^{[8
]}

Fich, Eric A. ^{[8
]}

Sun, Li ^{[9
,10
]}

Domozych, David S. ^{[9
,10
]}

Fraser, Paul D. ^{[6
]}

Tucker, Gregory A. ^{[1
]}

Grierson, Don ^{[1
]}

Seymour, Graham B. ^{[1
]}

机构：

[1] Univ Nottingham, Sch Biosci, Sutton Bonington Campus, Loughborough, Leics, England

[2] Bugbrooke Mills, Heygates Ltd, Bugbrooke, England

[3] Univ Nottingham, Sch Hlth Sci, Queens Med Ctr, Nottingham, England

[4] Jealotts Hill Int Res Stn, Syngenta Seeds, Bracknell, Berks, England

[5] Syngenta Crop Protect, Res Triangle Pk, NC USA

[6] Royal Holloway Univ London, Sch Biol Sci, Egham Hill, Egham, Surrey, England

[7] Univ Calif Davis, Plant Sci Dept, Davis, CA 95616 USA

[8] Cornell Univ, Plant Biol Sect, Sch Integrat Plant Sci, Ithaca, NY 14853 USA

[9] Skidmore Coll, Dept Biol, Saratoga Springs, NY 12866 USA

[10] Skidmore Coll, Skidmore Microscopy Imaging Ctr, Saratoga Springs, NY 12866 USA

[11] Springalls Farm, Valley Produce, Reading, Berks, England

[12] King Abdullah Univ Sci & Technol, Phys Sci & Engn Div, Thuwal, Saudi Arabia

[13] Q2 Solut, EA Genom, Morrisville, NC USA

来源：

NATURE BIOTECHNOLOGY | 2016年 / 34卷 / 09期

基金：

英国生物技术与生命科学研究理事会; 美国国家科学基金会; 英国工程与自然科学研究理事会;

关键词：

LYCOPERSICON-ESCULENTUM; POLYGALACTURONASE GENE; FRESH TOMATO; CELL-WALLS; EXPRESSION; HOMOGALACTURONAN; INHIBITION; REDUCTION;

D O I：

10.1038/nbt.3602

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Controlling the rate of softening to extend shelf life was a key target for researchers engineering genetically modified (GM) tomatoes in the 1990s, but only modest improvements were achieved. Hybrids grown nowadays contain 'non-ripening mutations' that slow ripening and improve shelf life, but adversely affect flavor and color. We report substantial, targeted control of tomato softening, without affecting other aspects of ripening, by silencing a gene encoding a pectate lyase.

引用

页码：950 / +

页数：6

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