Identification of intimin alleles in pathogenic Escherichia coli by PCR-restriction fragment length polymorphism analysis

被引:0
|
作者
C. Tramuta
P. Robino
E. Oswald
P. Nebbia
机构
[1] Università degli studi di Torino,Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia
[2] Facoltà di Medicina Veterinaria,undefined
[3] UMR960 INRA de Microbiologie Moleculaire,undefined
[4] Ecole Nationale Vétérinaire,undefined
来源
关键词
Intimin; PCR-RFLP;
D O I
暂无
中图分类号
学科分类号
摘要
A rapid two-step identification method based on PCR-RFLP analysis of the intimin gene was developed to differentiate specific alleles in pathogenic Escherichia coli. This technique, tested on isolates eae-positive, accurately detects eae and resolves alleles encoding the α1, α2, β, γ1, γ2/θ, κ, ɛ, ζ, and ι intimin variants.
引用
收藏
页码:1 / 5
页数:4
相关论文
共 50 条
  • [41] M protein typing of Thai group A streptococcal isolates by PCR-Restriction fragment length polymorphism analysis
    Yoonim, N
    Olive, C
    Pruksachatkunakorn, C
    Good, MF
    Pruksakorn, S
    BMC MICROBIOLOGY, 2005, 5 (1)
  • [42] Identification of Bartonella species directly in clinical specimens by PCR-restriction fragment length polymorphism analysis of a 16S rRNA gene fragment
    Matar, GM
    Koehler, JE
    Malcolm, G
    Lambert-Fair, MA
    Tappero, J
    Hunter, SB
    Swaminathan, B
    JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (12) : 4045 - 4047
  • [43] M protein typing of Thai group A streptococcal isolates by PCR-Restriction fragment length polymorphism analysis
    Nonglak Yoonim
    Colleen Olive
    Chulabhorn Pruksachatkunakorn
    Michael F Good
    Sumalee Pruksakorn
    BMC Microbiology, 5
  • [44] Usefulness of PCR-restriction fragment length polymorphism analysis of the internal transcribed Spacer region of rDNA for identification of Anisakis simplex complex
    Abe, N
    Tominaga, K
    Kimata, I
    JAPANESE JOURNAL OF INFECTIOUS DISEASES, 2006, 59 (01) : 60 - 62
  • [45] Molecular identification of Capnocytophaga spp. via 16S rRNA PCR-restriction fragment length polymorphism analysis
    Ciantar, M
    Newman, HN
    Wilson, M
    Spratt, DA
    JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (04) : 1894 - 1901
  • [46] An Improved PCR-Restriction Fragment Length Polymorphism (RFLP) Method for the Identification of cry1-Type Genes
    Shu, Changlong
    Liu, Dongming
    Zhou, Zishan
    Cai, Jilin
    Peng, Qi
    Gao, Jiguo
    Song, Fuping
    Zhang, Jie
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2013, 79 (21) : 6706 - 6711
  • [47] NUCLEOTIDE-SEQUENCE COMPARISON OF THE MYCOBACTERIAL DNAJ GENE AND PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS FOR IDENTIFICATION OF MYCOBACTERIAL SPECIES
    TAKEWAKI, S
    OKUZUMI, K
    MANABE, I
    TANIMURA, M
    MIYAMURA, K
    NAKAHARA, K
    YAZAKI, Y
    OHKUBO, A
    NAGAI, R
    INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1994, 44 (01): : 159 - 166
  • [48] MOLECULAR CHARACTERIZATION BY PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM OF TEM BETA-LACTAMASES
    ARLET, G
    BRAMI, G
    DECRE, D
    FLIPPO, A
    GAILLOT, O
    LAGRANGE, PH
    PHILIPPON, A
    FEMS MICROBIOLOGY LETTERS, 1995, 134 (2-3) : 203 - 208
  • [49] Rapid Identification of Mycobacteria from Smear-Positive Sputum Samples by Nested PCR-Restriction Fragment Length Polymorphism Analysis
    Wu, Tsu-Lan
    Chia, Ju-Hsin
    Kuo, An-Jing
    Su, Lin-Hui
    Wu, Ting-Shu
    Lai, Hsin-Chih
    JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (11) : 3591 - 3594
  • [50] Species-specific identification of Mycobacterium leprae by PCR-restriction fragment length polymorphism analysis of the hsp65 gene
    Rastogi, N
    Goh, KS
    Berchel, M
    JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (06) : 2016 - 2019