Identification of intimin alleles in pathogenic Escherichia coli by PCR-restriction fragment length polymorphism analysis

被引：0
|
作者：
C. Tramuta
P. Robino
E. Oswald
P. Nebbia
机构：
[1] Università degli studi di Torino,Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia
[2] Facoltà di Medicina Veterinaria,undefined
[3] UMR960 INRA de Microbiologie Moleculaire,undefined
[4] Ecole Nationale Vétérinaire,undefined
来源：
Veterinary Research Communications | 2008年 / 32卷
关键词：
Intimin; PCR-RFLP;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A rapid two-step identification method based on PCR-RFLP analysis of the intimin gene was developed to differentiate specific alleles in pathogenic Escherichia coli. This technique, tested on isolates eae-positive, accurately detects eae and resolves alleles encoding the α1, α2, β, γ1, γ2/θ, κ, ɛ, ζ, and ι intimin variants.
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页码：1 / 5
页数：4
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