Nutritional requirements for the production of pyrazoloisoquinolinone antibiotics by Streptomyces griseocarneus NCIMB 40447

This paper describes the effect of different nutrients on the production of pyrazoloisoquinolinone antibiotics (APHE) by Streptomyces griseocarneus. In a chemically defined medium with glucose as carbon and l-lysine as nitrogen source all APHE antibiotics (APHE-1 to -3) are produced, APHE-3 being the most abundant. Propionate and butyrate used as precursors with glucose as main carbon source increased the production of APHE-1 and -2, respectively. The presence of propionate or butyrate reduced the production of APHE-3. Results obtained in minimal medium supplemented with l-valine and l-histidine indicate a relationship between these amino acids and APHE biosynthesis. These data, together with those obtained in the presence of precursors of fatty acids, also show possible links with fatty acid biosynthesis. Different nutritional requirements were found for APHE-3 production in comparison with APHE-1 and APHE-2.