Extracellular production of a sphingomyelinase from Streptomyces griseocarneus using Streptomyces lividans

被引：5

作者：

Sugimori, Daisuke ^{[1
]}

Tomita, Yu ^{[1
]}

Matsumoto, Yusaku ^{[1
]}

Ogino, Chiaki ^{[2
]}

机构：

[1] Fukushima Univ, Dept Symbiot Syst Sci & Technol, Grad Sch Symbiot Syst Sci & Technol, Fukushima 9601296, Japan

[2] Kobe Univ, Grad Sch Technol, Dept Sci & Chem Engn, Kobe, Hyogo 6578501, Japan

来源：

BIOTECHNOLOGY LETTERS | 2011年 / 33卷 / 04期

关键词：

Mg(2+) enhancement of sphingomyelinase; Shuttle vector; Sphingomyelinase; Streptomyces griseocarneus; Streptomyces lividans; BACILLUS-CEREUS; NEUTRAL SPHINGOMYELINASE; PURIFICATION; EXPRESSION;

D O I：

10.1007/s10529-010-0480-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The structural gene for sphingomyelinase (SMase) from Streptomyces griseocarneus, was introduced into Streptomyces lividans using a shuttle vector, pUC702, for Escherichia coli/S. lividans. High-level secretory production of SMase was achieved using the promoter, signal sequence and terminator regions of phospholipase D from Streptoverticillium cinnamoneum. The transformant constitutively expressed a high specific activity of SMase extracellularly during batch culture. Maximum SMase activity (555 +/- A 114 U/mg protein) was with 1.75 M MgCl(2) which was about 50-fold more than that with 10 mM MgCl(2).

引用

页码：727 / 731

页数：5

共 50 条

[1] Extracellular production of a sphingomyelinase from Streptomyces griseocarneus using Streptomyces lividans
Daisuke Sugimori
Yu Tomita
Yusaku Matsumoto
Chiaki Ogino
Biotechnology Letters, 2011, 33 : 727 - 731
[2] Kinetic characterization and Mg2+ enhancement of Streptomyces griseocarneus sphingomyelinase C produced by recombinant Streptomyces lividans
Sugimori, Daisuke
Matsumoto, Yusaku
Tomita, Yu
Murayama, Kazutaka
Ogino, Chiaki
PROTEIN EXPRESSION AND PURIFICATION, 2012, 81 (02) : 151 - 156
[3] STUDIES ON STREPTOMYCES-GRISEOCARNEUS AND THE PRODUCTION OF HYDROXYSTREPTOMYCIN
BENEDICT, RG
LINDENFELSER, LA
STODOLA, FH
TRAUFLER, DH
JOURNAL OF BACTERIOLOGY, 1951, 62 (04) : 487 - 497
[4] A DNA fragment from Streptomyces fradiae increases the production of a metalloprotease in Streptomyces lividans
Mythili, S
Dharmalingam, K
JOURNAL OF BIOSCIENCES, 1997, 22 (04) : 419 - 430
[5] Structural basis for the high thermal stability and optimum pH of sphingomyelinase C from Streptomyces griseocarneus
Fujisawa, Ikuhide
Hamana, Hiroaki
Tomita, Yu
Matsumoto, Yusaku
Murayama, Kazutaka
Sugimori, Daisuke
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2021, 131 (01) : 8 - 12
[6] Purification, characterization, and gene cloning of sphingomyelinase C from Streptomyces griseocarneus NBRC13471
Sugimori, Daisuke
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2009, 108 (04) : 293 - 298
[7] Improved production of mannanase by Streptomyces lividans
Marga, F
Ghakis, C
Dupont, C
Morosoll, R
Kluepfel, D
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (12) : 4656 - 4658
[8] Heterologous production of daptomycin in Streptomyces lividans
Julia, P
Xiang, L
Whiting, A
Mohammed, L
Gibson, T
Silva, CJ
Brian, P
Davies, J
Miao, V
Wrigley, SK
Baltz, RH
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2006, 33 (02) : 121 - 128
[9] Novel aminoacylases from Streptomyces griseus DSM 40236 and their recombinant production in Streptomyces lividans
Haeger, Gerrit
Probst, Johanna
Jaeger, Karl-Erich
Bongaerts, Johannes
Siegert, Petra
FEBS OPEN BIO, 2023, 13 (12): : 2224 - 2238
[10] Optimal production conditions of Streptomyces griseus trypsin (SGT) in Streptomyces lividans
Koo, BJ
Kim, JM
Byun, SM
Hong, SK
JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1999, 32 (01): : 86 - 91

← 1 2 3 4 5 →