Extracellular production of a sphingomyelinase from Streptomyces griseocarneus using Streptomyces lividans

被引：0
|
作者：
Daisuke Sugimori
Yu Tomita
Yusaku Matsumoto
Chiaki Ogino
机构：
[1] Graduate School of Symbiotic Systems Science and Technology,Department of Symbiotic Systems Science and Technology
[2] Fukushima University,Department of Chemical Science and Engineering
[3] Graduate School of Technology,undefined
[4] Kobe University,undefined
来源：
Biotechnology Letters | 2011年 / 33卷
关键词：
Mg; enhancement of sphingomyelinase; Shuttle vector; Sphingomyelinase;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The structural gene for sphingomyelinase (SMase) from Streptomycesgriseocarneus, was introduced into Streptomyces lividans using a shuttle vector, pUC702, for Escherichia coli/S. lividans. High-level secretory production of SMase was achieved using the promoter, signal sequence and terminator regions of phospholipase D from Streptoverticillium cinnamoneum. The transformant constitutively expressed a high specific activity of SMase extracellularly during batch culture. Maximum SMase activity (555 ± 114 U/mg protein) was with 1.75 M MgCl2 which was about 50-fold more than that with 10 mM MgCl2.
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页码：727 / 731
页数：4
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