Extracellular production of a sphingomyelinase from Streptomyces griseocarneus using Streptomyces lividans

被引：5
|
作者：
Sugimori, Daisuke [1 ]
Tomita, Yu [1 ]
Matsumoto, Yusaku [1 ]
Ogino, Chiaki [2 ]
机构：
[1] Fukushima Univ, Dept Symbiot Syst Sci & Technol, Grad Sch Symbiot Syst Sci & Technol, Fukushima 9601296, Japan
[2] Kobe Univ, Grad Sch Technol, Dept Sci & Chem Engn, Kobe, Hyogo 6578501, Japan
来源：
BIOTECHNOLOGY LETTERS | 2011年 / 33卷 / 04期
关键词：
Mg(2+) enhancement of sphingomyelinase; Shuttle vector; Sphingomyelinase; Streptomyces griseocarneus; Streptomyces lividans; BACILLUS-CEREUS; NEUTRAL SPHINGOMYELINASE; PURIFICATION; EXPRESSION;
D O I：
10.1007/s10529-010-0480-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The structural gene for sphingomyelinase (SMase) from Streptomyces griseocarneus, was introduced into Streptomyces lividans using a shuttle vector, pUC702, for Escherichia coli/S. lividans. High-level secretory production of SMase was achieved using the promoter, signal sequence and terminator regions of phospholipase D from Streptoverticillium cinnamoneum. The transformant constitutively expressed a high specific activity of SMase extracellularly during batch culture. Maximum SMase activity (555 +/- A 114 U/mg protein) was with 1.75 M MgCl(2) which was about 50-fold more than that with 10 mM MgCl(2).
引用
收藏
页码：727 / 731
页数：5
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