Scanless two-photon excitation of channelrhodopsin-2

被引：0

作者：

Eirini Papagiakoumou

Francesca Anselmi

Aurélien Bègue

Vincent de Sars

Jesper Glückstad

Ehud Y Isacoff

Valentina Emiliani

机构：

[1] Wavefront-Engineering Microscopy Group,Department of Photonics Engineering

[2] Neurophysiology and New Microscopies Laboratory,Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute

[3] Centre National de la Recherche Scientifique,Material Science Division and Physical Bioscience Division

[4] Unité Mixte de Recherche 8154,undefined

[5] Institut National de la Santé et de la Recherche Médicale U603,undefined

[6] Paris Descartes University,undefined

[7] Technical University of Denmark (Fotonik),undefined

[8] University of California,undefined

[9] Berkeley,undefined

[10] Berkeley,undefined

[11] California,undefined

[12] USA.,undefined

[13] Lawrence Berkeley National Laboratory,undefined

来源：

Nature Methods | 2010年 / 7卷

关键词：

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中图分类号：

学科分类号：

摘要：

Generalized phase contrast and temporal focusing are combined to shape two-photon excitation patterns that elicit large photocurrents in ChR2-expressing neurons in culture and slices. This method allows precise aiming of the stimulating light at single neuronal processes, neurons or groups of neurons and can elicit simultaneous excitation of multiple cells using optogenetics.

引用

页码：848 / 854

页数：6

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