Scanless two-photon excitation of channelrhodopsin-2

被引：0
|
作者：
Eirini Papagiakoumou
Francesca Anselmi
Aurélien Bègue
Vincent de Sars
Jesper Glückstad
Ehud Y Isacoff
Valentina Emiliani
机构：
[1] Wavefront-Engineering Microscopy Group,Department of Photonics Engineering
[2] Neurophysiology and New Microscopies Laboratory,Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute
[3] Centre National de la Recherche Scientifique,Material Science Division and Physical Bioscience Division
[4] Unité Mixte de Recherche 8154,undefined
[5] Institut National de la Santé et de la Recherche Médicale U603,undefined
[6] Paris Descartes University,undefined
[7] Technical University of Denmark (Fotonik),undefined
[8] University of California,undefined
[9] Berkeley,undefined
[10] Berkeley,undefined
[11] California,undefined
[12] USA.,undefined
[13] Lawrence Berkeley National Laboratory,undefined
来源：
Nature Methods | 2010年 / 7卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Generalized phase contrast and temporal focusing are combined to shape two-photon excitation patterns that elicit large photocurrents in ChR2-expressing neurons in culture and slices. This method allows precise aiming of the stimulating light at single neuronal processes, neurons or groups of neurons and can elicit simultaneous excitation of multiple cells using optogenetics.
引用
收藏
页码：848 / 854
页数：6
相关论文
共 50 条
  • [21] Two-photon excitation of aluminium phthalocyanines
    Meshalkin, YP
    Alfimov, EE
    Vasil'ev, NE
    Denisov, AN
    Makukha, VK
    Ogirenko, AP
    QUANTUM ELECTRONICS, 1999, 29 (12) : 1066 - 1068
  • [22] Two-photon excitation of substituted enediynes
    Kauffman, JF
    Turner, JM
    Alabugin, IV
    Breiner, B
    Kovalenko, SV
    Badaeva, EA
    Masunov, A
    Tretiak, S
    JOURNAL OF PHYSICAL CHEMISTRY A, 2006, 110 (01): : 241 - 251
  • [23] Biphoton focusing for two-photon excitation
    Nasr, MB
    Abouraddy, AF
    Booth, MC
    Saleh, BEA
    Sergienko, AV
    Teich, MC
    Kempe, M
    Wolleschensky, R
    PHYSICAL REVIEW A, 2002, 65 (02): : 1 - 6
  • [24] Two-photon excitation of a phytofluor protein
    Gryczynski, I
    Piszczek, G
    Lakowicz, JR
    Lagarias, JC
    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 2002, 150 (1-3) : 13 - 19
  • [25] Photobleaching in two-photon excitation microscopy
    Patterson, GH
    Piston, DW
    BIOPHYSICAL JOURNAL, 2000, 78 (04) : 2159 - 2162
  • [26] Two-photon excitation STED microscopy
    Moneron, Gael
    Hell, Stefan W.
    OPTICS EXPRESS, 2009, 17 (17): : 14567 - 14573
  • [27] Two-photon excitation fluorescence bioassays
    Hanninen, Pekka
    Soukka, Jori
    Soini, Juhani T.
    FLUORESCENCE METHODS AND APPLICATIONS: SPECTROSCOPY, IMAGING, AND PROBES, 2008, 1130 : 320 - 326
  • [28] Lineshapes in coherent two-photon excitation
    Halfmann, T
    Rickes, T
    Vitanov, NV
    Bergmann, K
    OPTICS COMMUNICATIONS, 2003, 220 (4-6) : 353 - 359
  • [29] TWO-PHOTON EXCITATION IN PHENANTHRENE.
    Srivastava, G.P.
    Goyal, M.L.
    Shyam, Radhey
    Gupta, S.C.
    1972, 62 (06):
  • [30] Two-photon excitation of RPE melanin
    Glickman, RD
    Rockwell, BA
    Noojin, GD
    Stolarski, DJ
    Denton, ML
    Kumar, N
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2002, 43 : U148 - U148
12345