Evaluation of Enzymatic Activities in Living Systems with Small-molecular Fluorescent Substrate Probes

被引：0
|
作者：
Toru Komatsu
Yasuteru Urano
机构：
[1] The University of Tokyo,Graduate School of Pharmaceutical Sciences
[2] The University of Tokyo,Graduate School of Medicine
[3] JST PRESTO,undefined
来源：
Analytical Sciences | 2015年 / 31卷
关键词：
Enzymes; fluorescent substrates; fluorescence imaging; medicinal chemistry; chemical biology;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In this review, we aim to present an overview of how small-molecular fluorescent substrate probes for studying enzymatic functions are developed and how they are used in biological applications, under the following four headings: (1) History of Visual Detection of Enzymatic Activities, (2) Strategies to Design Fluorescent Substrate Probes to Measure Enzymatic Activities, (3) Development of Fluorescent Substrate Probes Suitable for Biological Studies, and (4) Biological Applications of Fluorescent Substrate Probes for Studying Enzymes.
引用
收藏
页码：257 / 265
页数：8
相关论文
共 50 条
  • [31] Tracking of Mitochondrial Endogenous Ribonucleic Acid in the Cancer Cells and Macrophages Using a Novel Small-Molecular Fluorescent Probe
    Liu, Yong
    Niu, Jie
    Wang, Weishan
    Lin, Weiying
    ANALYTICAL CHEMISTRY, 2019, 91 (03) : 1715 - 1718
  • [32] Fluorescent molecular probes V: A sensitive caspase-3 substrate for fluorometric assays
    Liu, JX
    Bhalgat, M
    Zhang, CL
    Diwu, ZJ
    Hoyland, B
    Klaubert, DH
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1999, 9 (22) : 3231 - 3236
  • [33] Unraveling Ferroptosis Mechanisms: Tracking Cellular Viscosity with Small Molecular Fluorescent Probes
    Pareek, Niharika
    Mendiratta, Sana
    Kalita, Nripankar
    Sivaramakrishnan, Shreya
    Khan, Rafique Sanu
    Samanta, Animesh
    CHEMISTRY-AN ASIAN JOURNAL, 2024, 19 (08)
  • [34] Small Molecular Fluorescent Probes for Alzheimer's Disease Associated Active Species
    Tang, Fuyan
    Wang, Kai
    Liu, Xueli
    Zhang, Xiaolei
    Zhou, Wanting
    Mu, Zonggao
    Zhang, Tian
    Shu, Wei
    Liu, Yuying
    Xiao, Haibin
    CHEMISTRY-A EUROPEAN JOURNAL, 2023, 29 (35)
  • [35] Development of Highly Sensitive Fluorescent Probes for Detection of Intracellular Copper(I) in Living Systems
    Taki, Masayasu
    Iyoshi, Shohei
    Ojida, Akio
    Hamachi, Itaru
    Yamamoto, Yukio
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (17) : 5938 - +
  • [36] Recent progress in the fluorescent probes for the specific imaging of small molecular weight thiols in living cells (vol 76, pg 166, 2016)
    Chen, Hua
    Tang, Yonghe
    Lin, Weiying
    TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2016, 77 : 242 - 242
  • [37] Activatable Molecular Systems Using Homologous Near-Infrared Fluorescent Probes for Monitoring Enzyme Activities in Vitro, in Cellulo, and in Vivo
    Zhang, Zongren
    Fan, Jinda
    Cheney, Philip P.
    Berezin, Mikhail Y.
    Edwards, W. Barry
    Akers, Walter J.
    Shen, Duanwen
    Liang, Kexian
    Culver, Joseph P.
    Achilefu, Samuel
    MOLECULAR PHARMACEUTICS, 2009, 6 (02) : 416 - 427
  • [38] Small molecule fluorescent probes for the detection of reactive nitrogen species in biological systems
    Zhou, Yongqing
    Yang, Xiaofeng
    Zhang, Jing
    Xu, Shuai
    Li, Juan
    Wang, Wenshou
    Yan, Mei
    COORDINATION CHEMISTRY REVIEWS, 2023, 493
  • [39] Synthesis and evaluation of turn-on fluorescent probes for imaging steroid sulfatase activities in cells
    Tai, Chih-Hsuan
    Lu, Chun-Ping
    Wu, Shih-Hsiung
    Lo, Lee-Chiang
    CHEMICAL COMMUNICATIONS, 2014, 50 (46) : 6116 - 6119
  • [40] Highly tunable small-molecule fluorescent probes to study protein misfolding in living cells
    Jun, Joo Myung
    Chenoweth, David
    Petersson, E.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2016, 252
12345