Evaluation of Enzymatic Activities in Living Systems with Small-molecular Fluorescent Substrate Probes

被引：0
|
作者：
Toru Komatsu
Yasuteru Urano
机构：
[1] The University of Tokyo,Graduate School of Pharmaceutical Sciences
[2] The University of Tokyo,Graduate School of Medicine
[3] JST PRESTO,undefined
来源：
Analytical Sciences | 2015年 / 31卷
关键词：
Enzymes; fluorescent substrates; fluorescence imaging; medicinal chemistry; chemical biology;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In this review, we aim to present an overview of how small-molecular fluorescent substrate probes for studying enzymatic functions are developed and how they are used in biological applications, under the following four headings: (1) History of Visual Detection of Enzymatic Activities, (2) Strategies to Design Fluorescent Substrate Probes to Measure Enzymatic Activities, (3) Development of Fluorescent Substrate Probes Suitable for Biological Studies, and (4) Biological Applications of Fluorescent Substrate Probes for Studying Enzymes.
引用
收藏
页码：257 / 265
页数：8
相关论文
共 50 条
  • [11] Molecular fluorescent probes for monitoring pH changes in living cells
    Wang, Rui
    Yu, Chunwei
    Yu, Fabiao
    Chen, Lingxin
    TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2010, 29 (09) : 1004 - 1013
  • [12] Genetically encoded betaxanthin-based small-molecular fluorescent reporter for mammalian cells
    Stucheli, Pascal
    Sieber, Simon
    Fuchs, David W.
    Scheller, Leo
    Strittmatter, Tobias
    Saxena, Pratik
    Gademann, Karl
    Fussenegger, Martin
    NUCLEIC ACIDS RESEARCH, 2020, 48 (12)
  • [13] Molecular fluorescent probes for imaging and evaluation of peroxynitrite fluctuations in living cells and in vivo under hypoxic stress
    Huang, Yan
    Yu, Lei
    Fu, Lili
    Hou, Junjun
    Wang, Lingxiao
    Sun, Mingzhao
    Wang, Xiaoyan
    Chen, Lingxin
    SENSORS AND ACTUATORS B-CHEMICAL, 2022, 370
  • [14] Molecular fluorescent probes for imaging and evaluation of peroxynitrite fluctuations in living cells and in vivo under hypoxic stress
    Huang, Yan
    Yu, Lei
    Fu, Lili
    Hou, Junjun
    Wang, Lingxiao
    Sun, Mingzhao
    Wang, Xiaoyan
    Chen, Lingxin
    SENSORS AND ACTUATORS B-CHEMICAL, 2022, 370
  • [15] Specific, cell-permeable fluorescent probes in the imaging of enzymatic pathways in living cells
    Reeves, Audrey
    Lippert, Alexander
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2016, 251
  • [16] Recent progresses in small-molecule enzymatic fluorescent probes for cancer imaging
    Liu, Hong-Wen
    Chen, Lanlan
    Xu, Chengyan
    Li, Zhe
    Zhang, Haiyang
    Zhang, Xiao-Bing
    Tan, Weihong
    CHEMICAL SOCIETY REVIEWS, 2018, 47 (18) : 7140 - 7180
  • [17] Recognition Moieties of Small Molecular Fluorescent Probes for Bioimaging of Enzymes
    Wu, Xiaofeng
    Shi, Wen
    Li, Xiaohua
    Ma, Huimin
    ACCOUNTS OF CHEMICAL RESEARCH, 2019, 52 (07) : 1892 - 1904
  • [18] The chronological evolution of small organic molecular fluorescent probes for thiols
    Yue, Yongkang
    Huo, Fangjun
    Yin, Caixia
    CHEMICAL SCIENCE, 2021, 12 (04) : 1220 - 1226
  • [19] Fluorescent molecular probes .1. The synthesis and biological properties of an ELF(R) beta-glucuronidase substrate that yields fluorescent precipitates at the enzymatic activity sites
    Diwu, Z
    Lu, YX
    Upson, RH
    Zhou, MJ
    Klaubert, DH
    Haugland, RP
    TETRAHEDRON, 1997, 53 (21) : 7159 - 7164
  • [20] BODIPY derivatives as fluorescent reporters of molecular activities in living cells
    Martynov, Vladimir, I
    Pakhomov, Alexey A.
    RUSSIAN CHEMICAL REVIEWS, 2021, 90 (10) : 1213 - 1262
12345