Development of a dot-blot system to detect gluten in food

被引：4

作者：

Gil, JR

Sbihi, Y

Alvarez, PA

Maache, M

Larrubia, M

Rojas, J

Osuna, A

机构：

[1] Univ Granada, Fac Ciencias, Dept Parasitol, Inst Biotecnol, E-18071 Granada, Spain

[2] Sanavi SA Lachar, Dept I&D&I, Granada, Spain

[3] Vircell SA Santa Fe, Dept I&D, Granada, Spain

[4] Dept Sci Vivant, Rabat, Morocco

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2003年 / 15卷 / 3-4期

关键词：

dot-blot; gluten; coeliac disease;

D O I：

10.1080/09540100400003576

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

We have designed a quick, easy, and reliable enzyme immunoassay system based on dot-blot methodology, capable of determining the presence of gluten or its derivatives in food. We also describe a new solution to extract the prolamin from flours. The sensitivity of the system is less than 5 ppm using a maximum volume of 2 mul.

引用

页码：235 / 242

页数：8

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