Development of a dot-blot system to detect gluten in food

被引：4
|
作者：
Gil, JR
Sbihi, Y
Alvarez, PA
Maache, M
Larrubia, M
Rojas, J
Osuna, A
机构：
[1] Univ Granada, Fac Ciencias, Dept Parasitol, Inst Biotecnol, E-18071 Granada, Spain
[2] Sanavi SA Lachar, Dept I&D&I, Granada, Spain
[3] Vircell SA Santa Fe, Dept I&D, Granada, Spain
[4] Dept Sci Vivant, Rabat, Morocco
来源：
FOOD AND AGRICULTURAL IMMUNOLOGY | 2003年 / 15卷 / 3-4期
关键词：
dot-blot; gluten; coeliac disease;
D O I：
10.1080/09540100400003576
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
We have designed a quick, easy, and reliable enzyme immunoassay system based on dot-blot methodology, capable of determining the presence of gluten or its derivatives in food. We also describe a new solution to extract the prolamin from flours. The sensitivity of the system is less than 5 ppm using a maximum volume of 2 mul.
引用
收藏
页码：235 / 242
页数：8
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