Solid phase subtractive cloning in differentially expressed genes identification

被引：1
|
作者：
D'Urso, Oscar F. [1 ]
D'Urso, Pietro I. [2 ]
Storelli, Carlo [3 ]
Marsigliante, Santo [3 ]
机构：
[1] Nonprofit Res Assoc, Promeridio Assoc, I-72018 San Michele Salentino, BR, Italy
[2] Univ Bari, Neurosurg Sect, Dept Neurosci, Sch Med, I-70124 Bari, Italy
[3] Univ Lecce, Dept Biol & Environm Sci & Technol DiSTeBA, I-73100 Lecce, Italy
来源：
MOLECULAR BIOLOGY REPORTS | 2010年 / 37卷 / 03期
关键词：
Subtractive cloning; Microarray; Solid surface; Library; Sequencing; Differentially expressed; CDNA; HYBRIDIZATION; CELLS;
D O I：
10.1007/s11033-009-9530-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We developed an array-based subtractive hybridization system for one-step high-throughput subtraction. We printed subtractor RNA up to 10.000 times obtaining an excellent contact surface using a little amount of RNA. During hybridization cDNA, common to subtractor and target samples, remains attached to slide immobilized RNA, leaving free in solution target specific cDNA which after retrieval is cloned.
引用
收藏
页码：1435 / 1438
页数：4
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