Role of Cigarette Smoke on Angiotensin-Converting Enzyme-2 Protein Membrane Expression in Bronchial Epithelial Cells Using an Air-Liquid Interface Model

被引:8
|
作者
Caruso, Massimo [1 ,2 ]
Distefano, Alfio [1 ]
Emma, Rosalia [1 ]
Di Rosa, Michelino [1 ]
Carota, Giuseppe [1 ]
Rust, Sonja [2 ]
Polosa, Riccardo [2 ,3 ]
Zuccarello, Pietro [4 ]
Ferrante, Margherita [2 ,4 ]
Raciti, Giuseppina [5 ]
Li Volti, Giovanni [1 ,2 ]
机构
[1] Univ Catania, Dept Biomed & Biotechnol Sci, Catania, Italy
[2] Univ Catania, Ctr Excellence Accelerat Harm Reduct CoEAHR, Catania, Italy
[3] Univ Catania, Dept Clin & Expt Med, Catania, Italy
[4] Univ Catania, Dept Med Surg Sci & Adv Technol GF Ingrassia, Catania, Italy
[5] Univ Catania, Dept Drug Sci, Catania, Italy
关键词
ACE-2; nicotine; smoke; cigarette; epithelial cells; GENE-EXPRESSION;
D O I
10.3389/fphar.2021.652102
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Prevalence studies of current smoking, among hospitalized COVID-19 patients, demonstrated an unexpectedly low prevalence among patients with COVID-19. The aim of the present study was to evaluate the effect of smoke from cigarettes on ACE-2 in bronchial epithelial cells. Normal bronchial epithelial cells (H292) were exposed to smoke by an air-liquid-interface (ALI) system and ACE-2 membrane protein expression was evaluated after 24 h from exposure. Our transcriptomics data analysis showed a significant selective reduction of membrane ACE-2 expression (about 25%) following smoking exposure. Interestingly, we observed a positive direct correlation between ACE-2 reduction and nicotine delivery. Furthermore, by stratifying GSE52237 as a function of ACE-2 gene expression levels, we highlighted 1,012 genes related to ACE-2 in smokers and 855 in non-smokers. Furthermore, we showed that 161 genes involved in the endocytosis process were highlighted using the online pathway tool KEGG. Finally, 11 genes were in common between the ACE-2 pathway in smokers and the genes regulated during endocytosis, while 12 genes with non-smokers. Interestingly, six in non-smokers and four genes in smokers were closely involved during the viral internalization process. Our data may offer a pharmaceutical role of nicotine as potential treatment option in COVID-19.
引用
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页数:12
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