DETECTION OF MICRO RIBONUCLEIC ACIDS FROM EXTRACTED EXTRACELLULAR VESICLES FOR CARDIOVASCULAR DISEASES BY USING AN INTEGRATED MICROFLUIDIC SYSTEM

被引：0
|
作者：
Cheng, Hong-Lin [1 ]
Fu, Chien-Yu [2 ]
Chen, Yen-Wen [1 ]
Wang, Yu-Lin [1 ,2 ]
Chen, Chih-Chen [1 ,2 ]
Lee, Gwo-Bin [1 ,2 ,3 ]
机构：
[1] Natl Tsing Hua Univ, Inst NanoEngn & MicroSyst, Hsinchu, Taiwan
[2] Natl Tsing Hua Univ, Dept Power Mech Engn, Hsinchu, Taiwan
[3] Natl Tsing Hua Univ, Inst Biomed Engn, Hsinchu, Taiwan
来源：
2018 IEEE MICRO ELECTRO MECHANICAL SYSTEMS (MEMS) | 2018年
关键词：
D O I：
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中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
Recently, micro ribonucleic acids (miRNAs) extracted from extracellular vesicles (EVs) have been recognized as promising biomarkers for early detection of cardiovascular diseases (CVDs). However, the detection and quantification of miRNAs by using the existing methods are relatively labor-intensive and time-consuming. In this work, an integrated microfluidic system equipped with highly sensitive field-effect transistors which is capable of performing EVs extraction, EVs lysis, target miRNAs extraction and miRNAs detection was reported. The entire detection process could be automated within three hours on a single chip and the detection limit of miRNAs was observed to be in a femtomolar range. which meets the requirement of physiological concentrations. This integrated microfluidic system has great potential to be a tool for early detection of CVDs.
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页码：1205 / 1208
页数：4
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