DETECTION OF MICRO RIBONUCLEIC ACIDS FROM EXTRACTED EXTRACELLULAR VESICLES FOR CARDIOVASCULAR DISEASES BY USING AN INTEGRATED MICROFLUIDIC SYSTEM

被引：0
|
作者：
Cheng, Hong-Lin [1 ]
Fu, Chien-Yu [2 ]
Chen, Yen-Wen [1 ]
Wang, Yu-Lin [1 ,2 ]
Chen, Chih-Chen [1 ,2 ]
Lee, Gwo-Bin [1 ,2 ,3 ]
机构：
[1] Natl Tsing Hua Univ, Inst NanoEngn & MicroSyst, Hsinchu, Taiwan
[2] Natl Tsing Hua Univ, Dept Power Mech Engn, Hsinchu, Taiwan
[3] Natl Tsing Hua Univ, Inst Biomed Engn, Hsinchu, Taiwan
来源：
2018 IEEE MICRO ELECTRO MECHANICAL SYSTEMS (MEMS) | 2018年
关键词：
D O I：
暂无
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
Recently, micro ribonucleic acids (miRNAs) extracted from extracellular vesicles (EVs) have been recognized as promising biomarkers for early detection of cardiovascular diseases (CVDs). However, the detection and quantification of miRNAs by using the existing methods are relatively labor-intensive and time-consuming. In this work, an integrated microfluidic system equipped with highly sensitive field-effect transistors which is capable of performing EVs extraction, EVs lysis, target miRNAs extraction and miRNAs detection was reported. The entire detection process could be automated within three hours on a single chip and the detection limit of miRNAs was observed to be in a femtomolar range. which meets the requirement of physiological concentrations. This integrated microfluidic system has great potential to be a tool for early detection of CVDs.
引用
收藏
页码：1205 / 1208
页数：4
相关论文
共 50 条
  • [31] AN INTEGRATED MICROFLUIDIC SYSTEM USING FIELD-EFFECT TRANSISTORS FOR CRP DETECTION
    Lin, Chih-Lin
    Kang, Yen-Wen
    Chang, Ko-Wei
    Chang, Wen-Hsin
    Wang, Yu-Lin
    Lee, Gwo-Bin
    2014 IEEE 27TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS), 2014, : 250 - 253
  • [32] Extracellular Vesicles Isolation from Large Volume Samples Using a Polydimethylsiloxane-Free Microfluidic Device
    Bajo-Santos, Cristina
    Priedols, Miks
    Kaukis, Pauls
    Paidere, Gunita
    Gerulis-Bergmanis, Romualds
    Mozolevskis, Gatis
    Abols, Arturs
    Rimsa, Roberts
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (09)
  • [33] Proteomic Profiling of Fallopian Tube-Derived Extracellular Vesicles Using a Microfluidic Tissue-on-Chip System
    Zha, Didi
    Rayamajhi, Sagar
    Sipes, Jared
    Russo, Angela
    Pathak, Harsh B. B.
    Li, Kailiang
    Sardiu, Mihaela E. E.
    Bantis, Leonidas E. E.
    Mitra, Amrita
    Puri, Rajni V. V.
    Trinidad, Camille V. V.
    Cain, Brian P. P.
    Isenberg, Brett C. C.
    Coppeta, Jonathan
    MacLaughlan, Shannon
    Godwin, Andrew K. K.
    Burdette, Joanna E. E.
    BIOENGINEERING-BASEL, 2023, 10 (04):
  • [34] Bacterial detection and identification from human synovial fluids on an integrated microfluidic system
    Liu, Ting-Hang
    Cheng, Shu-Shen
    You, Huey-Ling
    Lee, Mel S.
    Lee, Gwo-Bin
    ANALYST, 2019, 144 (04) : 1210 - 1222
  • [35] AN APTAMER BASED SANDWICH ASSAY FOR SIMULTANEOUS DETECTION OF MULTIPLE CARDIOVASCULAR BIOMARKERS ON A MULTILAYERED INTEGRATED MICROFLUIDIC SYSTEM
    Sinha, Anirban
    Gopinathan, Priya
    Chung, Yi-Da
    Shiesh, Shu-Chu
    Lee, Gwo-Bin
    2019 20TH INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS & EUROSENSORS XXXIII (TRANSDUCERS & EUROSENSORS XXXIII), 2019, : 1075 - 1077
  • [36] AUTOMATED CHARACTERIZATION OF CARDIOVASCULAR DISEASES USING WAVELET TRANSFORM FEATURES EXTRACTED FROM ECG SIGNALS
    Mohsin, Ahmad
    Faust, Oliver
    JOURNAL OF MECHANICS IN MEDICINE AND BIOLOGY, 2019, 19 (01)
  • [37] Detection of RAS mutations in colorectal cancer patients using DNA from extracellular vesicles
    Kuriyama, Sho
    Yamada, Takeshi
    Sonoda, Hiromichi
    Shinji, Seiichi
    Matsuda, Akihisa
    Yonaga, Kazuhide
    Iwai, Takuma
    Takeda, Kohki
    Ueda, Koji
    Miyasaka, Toshimitsu
    Kanaka, Shintaro
    Yoshida, Hiroshi
    CANCER RESEARCH, 2023, 83 (07)
  • [38] Automatic Authorship Detection Using Textual Patterns Extracted from Integrated Syntactic Graphs
    Gomez-Adorno, Helena
    Sidorov, Grigori
    Pinto, David
    Vilarino, Darnes
    Gelbukh, Alexander
    SENSORS, 2016, 16 (09):
  • [39] Author Correction: An integrated microfluidic system using a micro-fluxgate and micro spiral coil for magnetic microbeads trapping and detecting
    Xuecheng Sun
    Zhu Feng
    Shaotao Zhi
    Chong Lei
    Di Zhang
    Yong Zhou
    Scientific Reports, 8
  • [40] Detection of nanoparticles in mice using an integrated photoacoustic micro-ultrasound system
    Sun, John
    Heinmiller, Andrew
    Bates, Dave
    Needles, Andrew
    Theodoropoulos, Catherine
    FASEB JOURNAL, 2012, 26
12345