Species-Specific Type II Restriction-Modification System of Xylella fastidiosa Temecula1

被引：21
|
作者：
Matsumoto, Ayumi [1 ]
Igo, Michele M. [1 ]
机构：
[1] Univ Calif Davis, Dept Microbiol, Davis, CA 95616 USA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 12期
关键词：
IN-VITRO METHYLATION; TRANSFORMATION EFFICIENCY; ESCHERICHIA-COLI; PIERCES-DISEASE; DNA METHYLASE; CLONING; GENES; EXPRESSION; STRAINS; BARRIER;
D O I：
10.1128/AEM.03034-09
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The transformation efficiency of Xylella fastidiosa can be increased by interfering with restriction by the strain-specific type II system encoded by the PD1607 and PD1608 genes. Here, we report results for two strategies: in vitro methylation using M. SssI and isolation of DNA from an Escherichia coli strain expressing the methylase PD1607.
引用
收藏
页码：4092 / 4095
页数：4
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