Species-Specific Type II Restriction-Modification System of Xylella fastidiosa Temecula1

被引:21
|
作者
Matsumoto, Ayumi [1 ]
Igo, Michele M. [1 ]
机构
[1] Univ Calif Davis, Dept Microbiol, Davis, CA 95616 USA
关键词
IN-VITRO METHYLATION; TRANSFORMATION EFFICIENCY; ESCHERICHIA-COLI; PIERCES-DISEASE; DNA METHYLASE; CLONING; GENES; EXPRESSION; STRAINS; BARRIER;
D O I
10.1128/AEM.03034-09
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The transformation efficiency of Xylella fastidiosa can be increased by interfering with restriction by the strain-specific type II system encoded by the PD1607 and PD1608 genes. Here, we report results for two strategies: in vitro methylation using M. SssI and isolation of DNA from an Escherichia coli strain expressing the methylase PD1607.
引用
收藏
页码:4092 / 4095
页数:4
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