Optimizing the fluorescent yield in two-photon laser scanning microscopy with dispersion compensation

被引：27

作者：

Field, Jeffrey J. ^{[1
,2
]}

Carriles, Ramon ^{[1
,2
]}

Sheetz, Kraig E. ^{[1
,2
]}

Chandler, Eric V. ^{[1
,2
]}

Hoover, Erich E. ^{[1
,2
]}

Tillo, Shane E. ^{[3
]}

Hughes, Thom E. ^{[3
]}

Sylvester, Anne W. ^{[4
]}

Kleinfeld, David ^{[5
,6
]}

Squier, Jeff A. ^{[1
,2
]}

机构：

[1] Colorado Sch Mines, Ctr Microintegrated Opt Adv Bioimaging & Control, Golden, CO 80401 USA

[2] Colorado Sch Mines, Dept Phys, Golden, CO 80401 USA

[3] Montana State Univ, Dept Cell Biol & Neurosci, Bozeman, MT 59717 USA

[4] Univ Wyoming, Dept Mol Biol, Laramie, WY 82071 USA

[5] Univ Calif San Diego, Dept Phys, Grad Program Neurosci, La Jolla, CA 92093 USA

[6] Univ Calif San Diego, Ctr Neural Circuits & Behav, La Jolla, CA 92093 USA

来源：

OPTICS EXPRESS | 2010年 / 18卷 / 13期

基金：

美国国家科学基金会;

关键词：

ULTRASHORT PULSES; MULTIPHOTON; EFFICIENCY; PROTEIN; SIGNAL;

D O I：

10.1364/OE.18.013661

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A challenge for nonlinear imaging in living tissue is to maximize the total fluorescent yield from each fluorophore. We investigated the emission rates of three fluorophores-rhodamine B, a red fluorescent protein, and CdSe quantum dots-while manipulating the phase of the laser excitation pulse at the focus. In all cases a transform-limited pulse maximized the total yield to insure the highest signal-to-noise ratio. Further, we find evidence of fluorescence antibleaching in quantum dot samples. (C) 2010 Optical Society of America

引用

页码：13661 / 13672

页数：12

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