A Novel Fluorescent Protein Pair for Dual-color Two-photon Laser Scanning Microscopy

被引：0
|
作者：
Yang Song [2 ]
Teng Yan [1 ]
Xu Ping-Yong [1 ]
机构：
[1] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
[2] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China
来源：
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 2012年 / 39卷 / 10期
基金：
中国国家自然科学基金;
关键词：
two-photon; dual-color; fluorescent protein;
D O I：
10.3724/SP.J.1206.2012.00027
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Dual-color two-photon laser scanning microscopy is a useful method for simultaneously studying the expression, localization and trafficking of two different proteins in tissues. Because most two-photon microscopes only use a single wavelength excitation laser, simultaneously exciting multiple fluorescent proteins remains a challenge. Here, we present mAmetrine and mKate2, which can be used as a novel fluorescent protein pair in dual-color two-photon imaging by taking advantage of the large Stokes shift of mAmetrine and high brightness of mKate2. Both proteins have high two-photon absorption efficiencies and can be simultaneously excited at an optical wavelength of 765 nm. Dual-color two-photon imaging using this protein pair is highly effective in living cells.
引用
收藏
页码：1012 / 1016
页数：5
相关论文
共 12 条
  • [1] Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors
    Ai, Hui-wang
    Hazelwood, Kristin L.
    Davidson, Michael W.
    Campbell, Robert E.
    [J]. NATURE METHODS, 2008, 5 (05) : 401 - 403
  • [2] 2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY
    DENK, W
    STRICKLER, JH
    WEBB, WW
    [J]. SCIENCE, 1990, 248 (4951) : 73 - 76
  • [3] Absolute Two-Photon Absorption Spectra and Two-Photon Brightness of Orange and Red Fluorescent Proteins
    Drobizhev, M.
    Tillo, S.
    Makarov, N. S.
    Hughes, T. E.
    Rebane, A.
    [J]. JOURNAL OF PHYSICAL CHEMISTRY B, 2009, 113 (04): : 855 - 859
  • [4] Drobizhev M, 2011, NAT METHODS, V8, P393, DOI [10.1038/NMETH.1596, 10.1038/nmeth.1596]
  • [5] Two-photon dual-color imaging using fluorescent proteins
    Kawano, Hiroyuki
    Kogure, Takako
    Abe, Yukiko
    Mizuno, Hideaki
    Miyawaki, Atsushi
    [J]. NATURE METHODS, 2008, 5 (05) : 373 - 374
  • [6] A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy
    Kogure, T
    Karasawa, S
    Araki, T
    Saito, K
    Kinjo, M
    Miyawaki, A
    [J]. NATURE BIOTECHNOLOGY, 2006, 24 (05) : 577 - 581
  • [7] Li Dan, 2011, Zhonghua Yi Xue Za Zhi, V91, P1344
  • [8] Far-red fluorescent tags for protein imaging in living tissues
    Shcherbo, Dmitry
    Murphy, Christopher S.
    Ermakova, Galina V.
    Solovieva, Elena A.
    Chepurnykh, Tatiana V.
    Shcheglov, Aleksandr S.
    Verkhusha, Vladislav V.
    Pletnev, Vladimir Z.
    Hazelwood, Kristin L.
    Roche, Patrick M.
    Lukyanov, Sergey
    Zaraisky, Andrey G.
    Davidson, Michael W.
    Chudakov, Dmitriy M.
    [J]. BIOCHEMICAL JOURNAL, 2009, 418 : 567 - 574
  • [9] Principles of two-photon excitation microscopy and its applications to neuroscience
    Svoboda, Karel
    Yasuda, Ryohei
    [J]. NEURON, 2006, 50 (06) : 823 - 839
  • [10] A new approach to dual-color two-photon microscopy with fluorescent proteins
    Tillo, Shane E.
    Hughes, Thomas E.
    Makarov, Nikolay S.
    Rebane, Aleks
    Drobizhev, Mikhail
    [J]. BMC BIOTECHNOLOGY, 2010, 10
12