Optimizing the fluorescent yield in two-photon laser scanning microscopy with dispersion compensation

被引：27
|
作者：
Field, Jeffrey J. [1 ,2 ]
Carriles, Ramon [1 ,2 ]
Sheetz, Kraig E. [1 ,2 ]
Chandler, Eric V. [1 ,2 ]
Hoover, Erich E. [1 ,2 ]
Tillo, Shane E. [3 ]
Hughes, Thom E. [3 ]
Sylvester, Anne W. [4 ]
Kleinfeld, David [5 ,6 ]
Squier, Jeff A. [1 ,2 ]
机构：
[1] Colorado Sch Mines, Ctr Microintegrated Opt Adv Bioimaging & Control, Golden, CO 80401 USA
[2] Colorado Sch Mines, Dept Phys, Golden, CO 80401 USA
[3] Montana State Univ, Dept Cell Biol & Neurosci, Bozeman, MT 59717 USA
[4] Univ Wyoming, Dept Mol Biol, Laramie, WY 82071 USA
[5] Univ Calif San Diego, Dept Phys, Grad Program Neurosci, La Jolla, CA 92093 USA
[6] Univ Calif San Diego, Ctr Neural Circuits & Behav, La Jolla, CA 92093 USA
来源：
OPTICS EXPRESS | 2010年 / 18卷 / 13期
基金：
美国国家科学基金会;
关键词：
ULTRASHORT PULSES; MULTIPHOTON; EFFICIENCY; PROTEIN; SIGNAL;
D O I：
10.1364/OE.18.013661
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
A challenge for nonlinear imaging in living tissue is to maximize the total fluorescent yield from each fluorophore. We investigated the emission rates of three fluorophores-rhodamine B, a red fluorescent protein, and CdSe quantum dots-while manipulating the phase of the laser excitation pulse at the focus. In all cases a transform-limited pulse maximized the total yield to insure the highest signal-to-noise ratio. Further, we find evidence of fluorescence antibleaching in quantum dot samples. (C) 2010 Optical Society of America
引用
收藏
页码：13661 / 13672
页数：12
相关论文
共 50 条
  • [21] Multicolored two-photon fluorescent microscopy and localized two-photon fluorescent spectroscopy in living cells
    Bergey, EJ
    Wang, XPP
    Krebs, LJ
    Pudavar, HE
    Kapoor, R
    Friend, C
    Liebow, C
    Prasad, PN
    MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES, 2001, 4262 : 82 - 88
  • [22] Two-photon laser scanning microscopy integrated with light stimulus system
    Xu, Xin
    Jin, Xingjie
    Gao, Feng
    Shi, Guohua
    FIFTH SYMPOSIUM ON NOVEL OPTOELECTRONIC DETECTION TECHNOLOGY AND APPLICATION, 2019, 11023
  • [23] Two-photon excitation expands the capabilities of laser-scanning microscopy
    Manni, Jeff
    Biophotonics International, 1996, 3 (01):
  • [24] Microglial morphology determined with confocal and two-photon laser scanning microscopy
    Mitran, Smaranda Ioana
    Burada, Emilia
    Tanasie, Cornelia-Andreea
    Manea, Nicolae Catalin
    Ciorbagiu, Mihai Calin
    Mirea, Cecil Sorin
    Balseanu, Tudor-Adrian
    ROMANIAN JOURNAL OF MORPHOLOGY AND EMBRYOLOGY, 2018, 59 (02): : 485 - 490
  • [25] An alternative method for simulating IPSF in two-photon laser scanning microscopy
    Zhou, YJ
    Chen, DQ
    Huang, WH
    Xia, AD
    OPTICAL DESIGN AND TESTING, 2002, 4927 : 413 - 420
  • [26] Electrowetting prism for scanning in two-photon microscopy
    Supekar, Omkar D.
    Ozbay, Baris N.
    Zohrabi, Mo
    Nystrom, Philip D.
    Futia, Gregory L.
    Restrepo, Diego
    Gibson, Emily A.
    Gopinath, Juliet T.
    Bright, Victor M.
    2018 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO), 2018,
  • [27] Investigation of intact mouse cochleae using two-photon laser scanning microscopy
    Bae, Seong Hoon
    Kwak, Sang Hyun
    Choe, Young Ho
    Hyun, Young Min
    Choi, Jae Young
    Jung, Jinsei
    MICROSCOPY RESEARCH AND TECHNIQUE, 2020, 83 (10) : 1235 - 1240
  • [28] Applications and Prospects of Two-photon Laser Confocal Scanning Microscopy in Environmental Chemistry
    Wang Ping
    Zhu Ya-Xian
    Zhang Yong
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2008, 36 (10) : 1447 - 1452
  • [29] Longitudinal imaging of the taste bud in vivo with two-photon laser scanning microscopy
    Walters, Brittany N.
    Whiddon, Zachary D.
    Mcgee, Aaron W.
    Krimm, Robin F.
    PLOS ONE, 2024, 19 (12):
  • [30] Automatic dendritic spine analysis in two-photon laser scanning microscopy images
    Bai, Wenjia
    Zhou, Xiaobo
    Ji, Liang
    Cheng, Jie
    Wong, Stephen T. C.
    CYTOMETRY PART A, 2007, 71A (10) : 818 - 826
12345