Biochemical mechanisms of the RNA-induced silencing complex

被引：37
|
作者：
Paroo, Zain
Liu, Qinghua
Wang, Xiaodong
机构：
[1] Univ Texas, SW Med Ctr, Dept Biochem, Dallas, TX 75390 USA
[2] Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dallas, TX 75390 USA
来源：
CELL RESEARCH | 2007年 / 17卷 / 03期
关键词：
argonaute; Dicer; dsRBP; RISC; RNA interference; DOUBLE-STRANDED-RNA; MICRORNA-DEPENDENT LOCALIZATION; TARGETED MESSENGER-RNAS; C-ELEGANS; ENZYME COMPLEX; CAENORHABDITIS-ELEGANS; CRYSTAL-STRUCTURE; MAMMALIAN-CELLS; DISTINCT ROLES; HUMAN RISC;
D O I：
10.1038/sj.cr.7310148
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
In less than 10 years since its inception, RNA interference (RNAi) has had extraordinary impact on biomedical science. RNAI has been demonstrated to influence numerous biological and disease pathways. Development and adoption of RNAI technologies have been prolific ranging from basic loss-of-function tools, genome-wide screening libraries to pharmaceutical target validation and therapeutic development. However, understanding of the molecular mechanisms of RNAi is far from complete. The purpose of this brief review is to highlight key achievements in elucidating the biochemical mechanisms of the RNA-induced silencing complex and to outline major challenges for the field.
引用
收藏
页码：187 / 194
页数：8
相关论文
共 50 条
  • [21] Rational optimization of siRNA to ensure strand bias in the interaction with the RNA-induced silencing complex
    Datta, Dhrubajyoti
    Theile, Christopher S.
    Wassarman, Kelly
    Qin, June
    Racie, Tim
    Schmidt, Karyn
    Jiang, Yongfeng
    Sigel, Rachel
    Janas, Maja M.
    Egli, Martin
    Manoharan, Muthiah
    CHEMICAL COMMUNICATIONS, 2023, 59 (42) : 6347 - 6350
  • [22] Exploring plant genomes by RNA-induced gene silencing
    Peter M. Waterhouse
    Christopher A. Helliwell
    Nature Reviews Genetics, 2003, 4 : 29 - 38
  • [23] Capturing microRNA targets using an RNA-induced silencing complex (RISC)-trap approach
    Cambronne, Xiaolu A.
    Shen, Rongkun
    Auer, Paul L.
    Goodman, Richard H.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (50) : 20473 - 20478
  • [24] Exploring plant genomes by RNA-induced gene silencing
    Waterhouse, PM
    Helliwell, CA
    NATURE REVIEWS GENETICS, 2003, 4 (01) : 29 - 38
  • [25] Optimized protocols for RNA-induced silencing complex assembly and cleavage in cultured Drosophila cells
    Gao, Yajie
    Zhu, Yuanxiang
    Sun, Qinmiao
    Chen, Dahua
    STAR PROTOCOLS, 2022, 3 (03): : 101596
  • [26] RETRACTED: RNA editing of microRNA prevents RNA-induced silencing complex recognition of target mRNA (Retracted Article)
    Cui, Yalei
    Huang, Tianzhi
    Zhang, Xiaobo
    OPEN BIOLOGY, 2015, 5 (12)
  • [27] The RNA-induced transcriptional silencing complex targets chromatin exclusively via interacting with nascent transcripts
    Shimada, Yukiko
    Mohn, Fabio
    Buhler, Marc
    GENES & DEVELOPMENT, 2016, 30 (23) : 2571 - 2580
  • [28] Synaptotagmin 11 interacts with components of the RNA-induced silencing complex RISC in clonal pancreatic β-cells
    Milochau, Alexandra
    Lagree, Valerie
    Benassy, Marie-Noelle
    Chaignepain, Stephane
    Papin, Julien
    Garcia-Arcos, Itsaso
    Lajoix, Anne
    Monterrat, Carole
    Coudert, Laetitia
    Schmitter, Jean-Marie
    Ochoa, Begona
    Lang, Jochen
    FEBS LETTERS, 2014, 588 (14) : 2217 - 2222
  • [29] The MicroRNA and MessengerRNA Profile of the RNA-Induced Silencing Complex in Human Primary Astrocyte and Astrocytoma Cells
    Moser, Joanna J.
    Fritzler, Marvin J.
    PLOS ONE, 2010, 5 (10):
  • [30] ADAR1 Forms a Complex with Dicer to Promote MicroRNA Processing and RNA-Induced Gene Silencing
    Ota, Hiromitsu
    Sakurai, Masayuki
    Gupta, Ravi
    Valente, Louis
    Wulff, Bjorn-Erik
    Ariyoshi, Kentaro
    Iizasa, Hisashi
    Davuluri, Ramana V.
    Nishikura, Kazuko
    CELL, 2013, 153 (03) : 575 - 589
12345