Development of 17 Novel Cross-Species Microsatellite Markers for Dabry's Sturgeon (Acipenser dabryanus) from Chinese Sturgeon (Acipenser sinensis) via Next-Generation Sequencing

被引：2
|
作者：
Hu, Yacheng [1 ,2 ]
Liu, Xueqing [1 ,2 ]
Yang, Jing [1 ,2 ]
Xiao, Kan [1 ,2 ]
Wang, Binzhong [1 ,2 ]
Du, Hejun [1 ,2 ]
机构：
[1] China Three Gorges Corp, Chinese Sturgeon Res Inst, Yichang 443100, Hubei, Peoples R China
[2] Hubei Key Lab Three Gorges Project Conservat Fish, Yichang 443100, Hubei, Peoples R China
来源：
PAKISTAN JOURNAL OF ZOOLOGY | 2019年 / 51卷 / 06期
关键词：
Microsatellite; Acipenser dabryanus; Genetic diversity; Development; Cross-species; LOCI;
D O I：
10.17582/journal.pjz/2019.51.6.sc4
中图分类号：
Q95 [动物学];
学科分类号：
071002 ;
摘要：
In this study, we report 17 novel cross-species microsatellite markers for Dabry's sturgeon via next-generation sequencing from Chinese sturgeon (Acipenser sinensis). The 17 microsatellites were polymorphic with 3 to 6 alleles per locus and the total number of alleles is 80. The mean expected heterozygosity (H-E, 0.506 to 0.781), observed heterozygosity (H-O, 0.542 to 1), Hardy-Weinberg departure value (d, -0.042 to 0.427), polymorphic information content (PIC, 0.482 to 0.753) and the ShannonWiener Diversity Indices (H', 0.827 to 1.565) of all the 17 polymorphic loci indicated a high level of informativeness. Those markers will be useful tool to study the genetic diversity of Dabry's sturgeon.
引用
收藏
页码：2381 / 2384
页数：4
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