Development of 17 Novel Cross-Species Microsatellite Markers for Dabry's Sturgeon (Acipenser dabryanus) from Chinese Sturgeon (Acipenser sinensis) via Next-Generation Sequencing

被引:2
|
作者
Hu, Yacheng [1 ,2 ]
Liu, Xueqing [1 ,2 ]
Yang, Jing [1 ,2 ]
Xiao, Kan [1 ,2 ]
Wang, Binzhong [1 ,2 ]
Du, Hejun [1 ,2 ]
机构
[1] China Three Gorges Corp, Chinese Sturgeon Res Inst, Yichang 443100, Hubei, Peoples R China
[2] Hubei Key Lab Three Gorges Project Conservat Fish, Yichang 443100, Hubei, Peoples R China
关键词
Microsatellite; Acipenser dabryanus; Genetic diversity; Development; Cross-species; LOCI;
D O I
10.17582/journal.pjz/2019.51.6.sc4
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
In this study, we report 17 novel cross-species microsatellite markers for Dabry's sturgeon via next-generation sequencing from Chinese sturgeon (Acipenser sinensis). The 17 microsatellites were polymorphic with 3 to 6 alleles per locus and the total number of alleles is 80. The mean expected heterozygosity (H-E, 0.506 to 0.781), observed heterozygosity (H-O, 0.542 to 1), Hardy-Weinberg departure value (d, -0.042 to 0.427), polymorphic information content (PIC, 0.482 to 0.753) and the ShannonWiener Diversity Indices (H', 0.827 to 1.565) of all the 17 polymorphic loci indicated a high level of informativeness. Those markers will be useful tool to study the genetic diversity of Dabry's sturgeon.
引用
收藏
页码:2381 / 2384
页数:4
相关论文
共 50 条
  • [21] Next-Generation Sequencing to Determine Changes in the Intestinal Microbiome of Juvenile Sturgeon Hybrid (Acipenser gueldenstaedtii♀ x Acipenser baerii♂) Resulting from Sodium Butyrate, Β-Glucan and Vitamin Supplementation
    Arciuch-Rutkowska, Martyna
    Nowosad, Joanna
    Luczynski, Michal Krzysztof
    Hussain, Syed Makhdoom
    Kucharczyk, Dariusz
    GENES, 2024, 15 (10)
  • [22] Development and characterization of novel polymorphic microsatellite markers for the Korean freshwater snail Semisulcospira coreana and cross-species amplification using next-generation sequencing
    Yeon Jung PARK
    Mi Nan LEE
    Eun-Mi KIM
    Jung Youn PARK
    JaeKoo NOH
    Tae-Jin CHOI
    Jung-Ha KANG
    JournalofOceanologyandLimnology, 2020, 38 (02) : 503 - 508
  • [23] Development and characterization of novel polymorphic microsatellite markers for the Korean freshwater snail Semisulcospira coreana and cross-species amplification using next-generation sequencing
    Park, Yeon Jung
    Lee, Mi Nan
    Kim, Eun-Mi
    Park, Jung Youn
    Noh, Jae Koo
    Choi, Tae-Jin
    Kang, Jung-Ha
    JOURNAL OF OCEANOLOGY AND LIMNOLOGY, 2020, 38 (02) : 503 - 508
  • [24] Development and characterization of novel polymorphic microsatellite markers for the Korean freshwater snail Semisulcospira coreana and cross-species amplification using next-generation sequencing
    Yeon Jung Park
    Mi Nan Lee
    Eun-Mi Kim
    Jung Youn Park
    Jae Koo Noh
    Tae-Jin Choi
    Jung-Ha Kang
    Journal of Oceanology and Limnology, 2020, 38 : 503 - 508
  • [25] Isolation and characterization of microsatellite loci in the Persian sturgeon (Acipenser persicus, Borodine, 1897) and cross-species amplification in four commercial sturgeons from the Caspian Sea
    Moghim, M.
    Heist, E. J.
    Tan, S. G.
    Pourkazemi, M.
    Siraj, S. S.
    Panandam, J. M.
    Pourgholam, R.
    Kor, D.
    Laloei, F.
    Taghavi, M. J.
    IRANIAN JOURNAL OF FISHERIES SCIENCES, 2012, 11 (03): : 548 - 558
  • [26] Isolation and characterization of microsatellite markers in Garcinia gummi-gutta by next-generation sequencing and cross-species amplification
    Ravishankar, K. V.
    Vasudeva, R.
    Hemanth, Byatroy
    Sandya, B. S.
    Sthapit, B. R.
    Parthasarathy, V. A.
    Rao, V. Ramanatha
    JOURNAL OF GENETICS, 2017, 96 (02) : 213 - 218
  • [27] Isolation and characterization of microsatellite markers in Garcinia gummi-gutta by next-generation sequencing and cross-species amplification
    K. V. RAVISHANKAR
    R. VASUDEVA
    BYATROY HEMANTH
    B. S. SANDYA
    B. R. STHAPIT
    V. A. PARTHASARATHY
    V. RAMANATHA RAO
    Journal of Genetics, 2017, 96 : 213 - 218
  • [28] Rapid Development of Microsatellite Markers for Plantago ovata Forsk.: Using Next Generation Sequencing and Their Cross-Species Transferability
    Fougat, Ranbir Singh
    Joshi, Chaitanya
    Kulkarni, Kalyani
    Kumar, Sushil
    Patel, Anand
    Sakure, Amar
    Mistry, Jigar
    AGRICULTURE-BASEL, 2014, 4 (02): : 199 - 216
  • [29] Cross-Species, Amplifiable Microsatellite Markers for Neoverrucid Barnacles from Deep-Sea Hydrothermal Vents Developed Using Next-Generation Sequencing
    Nakajima, Yuichi
    Shinzato, Chuya
    Khalturina, Mariia
    Watanabe, Hiromi
    Inagaki, Fumio
    Satoh, Nori
    Mitarai, Satoshi
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2014, 15 (08) : 14364 - 14371
  • [30] Isolation and characterization of microsatellite markers from Garcinia morella using next generation sequencing technology and cross-species amplification
    Ravishankar, K. V.
    Vasudeva, R.
    Hemanth, B.
    Narendrababu, D.
    Nischita, P.
    Sandya, B. S.
    Sthapit, B. R.
    Parthasarathy, V. A.
    Rao, V. Ramanatha
    JOURNAL OF HORTICULTURAL SCIENCES, 2023, 18 (02): : 338 - 344